FlowCam 8400以及通过成像流式细胞术对蓝藻浮游植物进行分类和活力染色
FlowCam 8400 and FlowCam Cyano Phytoplankton Classification and Viability Staining by Imaging Flow Cytometry.
作者信息
Roache-Johnson Kathryn H, Stephens Nicole R
机构信息
Yokogawa Fluid Imaging Technologies, Scarborough, ME, USA.
出版信息
Methods Mol Biol. 2023;2635:219-244. doi: 10.1007/978-1-0716-3020-4_13.
This chapter provides a protocol for a detailed evaluation of phytoplankton and nuisance cyanobacteria with the FlowCam 8400 and the FlowCam Cyano. The chapter includes (i) detailed description of the quality control of fluorescent mode of the FlowCam, (ii) detailing methods for discriminating nuisance cyanobacteria using the FlowCam Cyano, how to set up libraries and classification routines for commonly used classification reports, and (iii) detailing methods for viability staining to quantify LIVE versus DEAD phytoplankton using the FlowCam 8400.
本章提供了一种使用FlowCam 8400和FlowCam Cyano对浮游植物和有害蓝藻进行详细评估的方案。本章包括：(i) FlowCam荧光模式质量控制的详细描述；(ii) 详细说明使用FlowCam Cyano鉴别有害蓝藻的方法、如何为常用分类报告设置库和分类程序；以及(iii) 详细说明使用FlowCam 8400进行活力染色以量化活浮游植物与死浮游植物的方法。
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