Weiss Luisa, MacLeod Hayley, Comer Shane P, Cullivan Sarah, Szklanna Paulina B, Ní Áinle Fionnuala, Kevane Barry, Maguire Patricia B
UCD Conway SPHERE Research Group, Conway Institute, University College Dublin, Dublin, Ireland; School of Biomolecular and Biomedical Science, University College Dublin, Dublin, Ireland.
Department of Respiratory Medicine, Mater Misericordiae University Hospital, Dublin, Ireland.
STAR Protoc. 2023 Apr 18;4(2):102150. doi: 10.1016/j.xpro.2023.102150.
The contents of the platelet releasate (PR) play significant roles in hemostasis, inflammation, and pathologic sequelae. Careful platelet isolation to ensure quiescence and subsequent activation is key to the successful generation of PR. Here, we describe steps to isolate and aggregate quiescent washed platelets from whole blood of a clinical patient cohort. We then detail the generation of PR from isolated human washed platelets under clinical conditions. This protocol allows the investigation of platelet cargoes released through various activation pathways.
血小板释放物（PR）的成分在止血、炎症和病理后遗症中发挥着重要作用。仔细分离血小板以确保其静止状态并随后激活，是成功生成PR的关键。在此，我们描述了从临床患者队列的全血中分离和聚集静止洗涤血小板的步骤。然后，我们详细说明了在临床条件下从分离的人洗涤血小板中生成PR的过程。该方案允许研究通过各种激活途径释放的血小板货物。
