Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic.
Department of Pharmacology and Pharmacy, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic.
Sci Rep. 2023 Apr 19;13(1):6419. doi: 10.1038/s41598-023-33575-4.
There are many advantages associated with the determination of the level of corticosterone in rabbits from saliva, since this is a non-invasive sample collection method that does not affect their welfare and provides a reliable reflection of the state of the animal at a given moment without the results being distorted as they may be, for example, when blood samples are taken. The aim of this study was to determine the diurnal rhythm in the concentration of corticosterone in the saliva of the domestic rabbit. Saliva samples were taken from six domestic rabbits five times during the daytime (at 6:00, 9:00, 12:00, 15:00 and 18:00) over the course of three consecutive days. The levels of corticosterone in the saliva of the individual rabbits displayed a diurnal rhythm during the course of the day, with a significant increase between 12:00 and 15:00 (p < 0.05). No statistically significant difference in the concentrations of corticosterone in the saliva of the individual rabbits was demonstrated. Although the basal value of corticosterone is not known in rabbits and is difficult to determine, the results of our study show the pattern of fluctuations in the concentration of corticosterone in the saliva of rabbits during the daytime.
从唾液中测定皮质酮水平对兔子有许多好处，因为这是一种非侵入性的样本采集方法，不会影响它们的福利，并能可靠地反映动物在特定时刻的状态，而不会像采血样那样导致结果失真。本研究旨在确定家兔唾液中皮质酮浓度的昼夜节律。在连续三天的时间里，从六只家兔身上采集了五次唾液样本（分别在 6:00、9:00、12:00、15:00 和 18:00 采集）。在一天的过程中，个体兔子的唾液皮质酮水平呈现出昼夜节律，在 12:00 到 15:00 之间有显著增加（p<0.05）。个体兔子唾液中皮质酮浓度没有表现出统计学上的显著差异。虽然兔子的基础皮质酮值未知且难以确定，但我们的研究结果显示了兔子白天唾液中皮质酮浓度波动的模式。
