Prabhudesai Sonal A, Carvalho Karla, Dhupar Anita, Spadigam Anita
Department of Oral and Maxillofacial Pathology, Goa Dental College and Hospital, Bambolim, Goa, India.
Indian J Pathol Microbiol. 2023 Apr-Jun;66(2):332-338. doi: 10.4103/ijpm.ijpm_512_21.
The extracellular matrix (ECM) is a dynamic tissue that provides nutrition and support to overlying epithelium. During tumorigenesis, the tumor microenvironment (TME) dysregulates the ECM. This is reflected by morphological changes seen in collagen and elastic fibers and is thought to facilitate metastasis.
To study the degradation of elastic fibers in different grades of oral squamous cell carcinoma (OSCC) and in oral epithelial dysplasia (OED) using histochemistry and to correlate it to the TNM stage of OSCC.
Tumor cores from 38 cases of OSCC (well-differentiated, moderately differentiated, and poorly differentiated) and 15 incisional biopsies of OED were analyzed. Hematoxylin-eosin and Verhoeff's-Van Gieson (VVG) stains were used. The stained sections were assessed for morphological changes in elastic fibers.
Data were analyzed using Statistical Package for Social Sciences (SPSS) version 22 software. Fisher's exact, Kruskal-Wallis, one-way ANOVA, and Turkey post hoc tests were used to establish significance (P ≤ 0.05). Spearman's correlation test was used to correlate elastin fiber degradation with TNM stage of OSCC.
All grades of OSCC showed absence of elastic fibers around the tumor islands. Elastic fiber degradation (fragmented and clumped type fibers) increased proportionately with the grade and TNM stage of OSCC. In OED, A significant reduction in the amount of elastic fibers with increasing grade was noted.
A positive correlation was noted between elastin degradation and grade and stage of OSCC. Therefore, it may be implicated in tumor progression of OSCC.
细胞外基质（ECM）是一种动态组织，为覆盖其上的上皮提供营养和支持。在肿瘤发生过程中，肿瘤微环境（TME）会使ECM失调。这表现为胶原蛋白和弹性纤维的形态变化，被认为有助于转移。
利用组织化学研究不同分级的口腔鳞状细胞癌（OSCC）和口腔上皮发育异常（OED）中弹性纤维的降解情况，并将其与OSCC的TNM分期相关联。
分析了38例OSCC（高分化、中分化和低分化）的肿瘤核心以及15例OED的切取活检标本。使用苏木精-伊红染色和Verhoeff氏-范吉森（VVG）染色。对染色切片评估弹性纤维的形态变化。
使用社会科学统计软件包（SPSS）22版软件分析数据。采用Fisher精确检验、Kruskal-Wallis检验、单因素方差分析和Turkey事后检验确定显著性（P≤0.05）。使用Spearman相关性检验将弹性纤维降解与OSCC的TNM分期相关联。
所有分级的OSCC肿瘤岛周围均未见弹性纤维。弹性纤维降解（碎片化和聚集型纤维）与OSCC的分级和TNM分期成比例增加。在OED中，随着分级增加，弹性纤维数量显著减少。
弹性蛋白降解与OSCC的分级和分期之间存在正相关。因此，它可能与OSCC的肿瘤进展有关。
