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鼠伤寒沙门氏菌中假尿苷酸合成酶I的纯化

Purification of pseudouridylate synthetase I from Salmonella typhimurium.

作者信息

Arena F, Ciliberto G, Ciampi S, Cortese R

出版信息

Nucleic Acids Res. 1978 Dec;5(12):4523-36. doi: 10.1093/nar/5.12.4523.

DOI:10.1093/nar/5.12.4523
PMID:370771
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342770/
Abstract

Pseudouridylate synthetase from Salmonella typhimurium has been purified 1,000 fold and is about 90% pure. The enzyme has a molecular weight of 50,000 daltons. In the presence of tRNA there is a change in molecular weight from 50.000 to 100.000. This change does not seem to be due to the formation of a tRNA-enzyme complex but rather to a tRNA induced dimerization. Other properties of the enzyme are described.

摘要

鼠伤寒沙门氏菌的假尿苷酸合成酶已被纯化了1000倍，纯度约为90%。该酶的分子量为50,000道尔顿。在tRNA存在的情况下，分子量从50,000变为100,000。这种变化似乎不是由于形成了tRNA - 酶复合物，而是由于tRNA诱导的二聚化。文中还描述了该酶的其他特性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7930/342770/dd41a0c265dd/nar00473-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7930/342770/dd41a0c265dd/nar00473-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7930/342770/dd41a0c265dd/nar00473-0060-a.jpg

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本文引用的文献

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Temperature sensitive mutants of Escherichia coli for tRNA synthesis.用于tRNA合成的大肠杆菌温度敏感突变体。
Nucleic Acids Res. 1974 Mar;1(3):355-71. doi: 10.1093/nar/1.3.355.
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Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
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Separation of transfer ribonucleic acids by reverse phase chromatography.通过反相色谱法分离转移核糖核酸
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hisT is part of a multigene operon in Escherichia coli K-12.hisT是大肠杆菌K-12中一个多基因操纵子的一部分。
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Altered growth-rate-dependent regulation of 6-phosphogluconate dehydrogenase level in hisT mutants of Salmonella typhimurium and Escherichia coli.鼠伤寒沙门氏菌和大肠杆菌hisT突变体中6-磷酸葡萄糖酸脱氢酶水平的生长速率依赖性调节改变
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Absence of hisT-mediated tRNA pseudouridylation results in a uracil requirement that interferes with Escherichia coli K-12 cell division.组氨酸介导的tRNA假尿苷化缺失导致尿嘧啶需求，这会干扰大肠杆菌K-12的细胞分裂。
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Sequence and structure requirements for the biosynthesis of pseudouridine (psi 35) in plant pre-tRNA(Tyr).植物前体tRNA（Tyr）中假尿苷（ψ35）生物合成的序列和结构要求
EMBO J. 1992 May;11(5):1907-12. doi: 10.1002/j.1460-2075.1992.tb05243.x.
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Behavior of the nerve growth factor during electrofocusing.
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Isolation of mutants of Escherichia coli lac king 5-methyluracil in transfer ribonucleic acid or 1-methylguanine in ribosomal RNA.分离大肠杆菌转移核糖核酸中缺乏5-甲基尿嘧啶或核糖体核糖核酸中缺乏1-甲基鸟嘌呤的突变体。
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The nucleotide sequence of a threonine transfer ribonucleic acid from Escherichia coli.来自大肠杆菌的苏氨酸转移核糖核酸的核苷酸序列。
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Primary structure of E. coli alanine transfer RNA: relation to the yeast phenylalanyl tRNA synthetase recognition site.大肠杆菌丙氨酸转移核糖核酸的一级结构：与酵母苯丙氨酰转移核糖核酸合成酶识别位点的关系。
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Biosynthesis of pseudouridine in transfer ribonucleic acid.转运核糖核酸中假尿苷的生物合成
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Mutants of Escherichia coli thermosensitive for the synthesis of transfer RNA.对转运RNA合成具有温度敏感性的大肠杆菌突变体。
Proc Natl Acad Sci U S A. 1973 Jul;70(7):2091-5. doi: 10.1073/pnas.70.7.2091.
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Mutant tRNA His ineffective in repression and lacking two pseudouridine modifications.突变型组氨酸转运RNA在抑制方面无效且缺乏两个假尿苷修饰。
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