鼠伤寒沙门氏菌中假尿苷酸合成酶I的纯化
Purification of pseudouridylate synthetase I from Salmonella typhimurium.
作者信息
Arena F, Ciliberto G, Ciampi S, Cortese R
出版信息
Nucleic Acids Res. 1978 Dec;5(12):4523-36. doi: 10.1093/nar/5.12.4523.
Abstract
Pseudouridylate synthetase from Salmonella typhimurium has been purified 1,000 fold and is about 90% pure. The enzyme has a molecular weight of 50,000 daltons. In the presence of tRNA there is a change in molecular weight from 50.000 to 100.000. This change does not seem to be due to the formation of a tRNA-enzyme complex but rather to a tRNA induced dimerization. Other properties of the enzyme are described.
摘要
鼠伤寒沙门氏菌的假尿苷酸合成酶已被纯化了1000倍，纯度约为90%。该酶的分子量为50,000道尔顿。在tRNA存在的情况下，分子量从50,000变为100,000。这种变化似乎不是由于形成了tRNA - 酶复合物，而是由于tRNA诱导的二聚化。文中还描述了该酶的其他特性。
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