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大肠杆菌核糖体RNA操纵子中异亮氨酸tRNA1基因及其周边区域的序列

Sequence of the gene for isoleucine tRNA1 and the surrounding region in a ribosomal RNA operon of Escherichia coli.

作者信息

Sekiya T, Nishimura S

出版信息

Nucleic Acids Res. 1979 Feb;6(2):575-92. doi: 10.1093/nar/6.2.575.

DOI:10.1093/nar/6.2.575
PMID:370791
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327714/
Abstract

A DNA fragment of about 2000 base pairs carrying the gene for tRNA(1) (Ile) has been cloned from a total Eco RI endonuclease digest of Escherichia coli DNA. Sequence analyses revealed that about the first 850 base pairs from one end of the fragment contain a nucleotide sequence corresponding to that in the 3'-end of 16S rRNA. The gene for tRNA(Ile) follows the 16S rRNA gene and both genes flank a spacer sequence of 68 base pairs. The spacer region contains a repeating, a hair pin and a symmetrical structure when the sequence is viewed in the single stranded form. A notable hair pin structure is also observed in the region adjacent to the 3'-end of the tRNA(1) (Ile) gene. In addition, about 850 base pairs from the other end of the DNA fragment have been found to contain the nucleotide sequence of the 5'-end of 23S rRNA. The presence of the genes for tRNA(1) (Ile), 16S and 23S rRNA and the hybridization to tRNA(1) (Ala) suggest that this cloned DNA is part of one of the E. coli rRNA operons carrying these two tRNA genes as a spacer.Images

摘要

一段携带tRNA(1)（Ile）基因的约2000个碱基对的DNA片段已从大肠杆菌DNA的全Eco RI核酸内切酶消化产物中克隆出来。序列分析表明，该片段一端约前850个碱基对包含与16S rRNA 3'-端相对应的核苷酸序列。tRNA（Ile）基因紧跟在16S rRNA基因之后，这两个基因两侧是一个68个碱基对的间隔序列。当以单链形式查看序列时，间隔区域包含一个重复结构、一个发夹结构和一个对称结构。在tRNA(1)（Ile）基因3'-端相邻区域也观察到一个显著的发夹结构。此外，已发现DNA片段另一端约850个碱基对包含23S rRNA 5'-端的核苷酸序列。tRNA(1)（Ile）、16S和23S rRNA基因的存在以及与tRNA(1)（Ala）的杂交表明，这个克隆的DNA是大肠杆菌rRNA操纵子之一的一部分，该操纵子携带这两个tRNA基因作为间隔序列。图片

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/327714/788b0ca9a797/nar00443-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/327714/8098a18b9264/nar00443-0163-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/327714/b8ac35efab45/nar00443-0164-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/327714/788b0ca9a797/nar00443-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/327714/8098a18b9264/nar00443-0163-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/327714/b8ac35efab45/nar00443-0164-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4582/327714/788b0ca9a797/nar00443-0166-a.jpg

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本文引用的文献

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Nat New Biol. 1971 Sep 8;233(36):35-9. doi: 10.1038/newbio233035a0.
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The sequence of nucleotides in tRNA Ile from E. coli B.来自大肠杆菌B的异亮氨酸转运RNA（tRNA Ile）中的核苷酸序列。
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A pseudogene cluster in the leader region of the Euglena chloroplast 16S-23S rRNA genes.眼虫叶绿体16S - 23S rRNA基因前导区域中的一个假基因簇。
Nucleic Acids Res. 1982 Mar 11;10(5):1771-80. doi: 10.1093/nar/10.5.1771.
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The nucleotide sequence of the maize and spinach chloroplast isoleucine transfer RNA encoded in the 16S to 23S rDNA spacer.编码于16S至23S核糖体DNA间隔区的玉米和菠菜叶绿体异亮氨酸转移RNA的核苷酸序列。
Nucleic Acids Res. 1982 Mar 11;10(5):1653-9. doi: 10.1093/nar/10.5.1653.
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Sequence of the distal tRNA1Asp gene and the transcription termination signal in the Escherichia coli ribosomal RNA operon rrnF(or G).大肠杆菌核糖体RNA操纵子rrnF（或G）中远端tRNA1Asp基因的序列及转录终止信号
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