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GTSE1 通过 ERK/MAPK 通路调节微管相关蛋白促进 NSCLC 的生长。

GTSE1 promotes the growth of NSCLC by regulating microtubule-associated proteins through the ERK/MAPK pathway.

机构信息

Department of Clinical Nutrition, Yunnan Cancer Hospital/The Third Affiliated Hospital of Kunming Medical University, Kunming, China.

Department of Clinical Nutrition, Yunnan Cancer Center, Kunming, China.

出版信息

Thorac Cancer. 2023 Jun;14(17):1624-1634. doi: 10.1111/1759-7714.14908. Epub 2023 Apr 20.

DOI:10.1111/1759-7714.14908
PMID:37079439
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10260487/
Abstract

The role of G2 and S phase-expressed-1 (GTSE1), a microtubule-localized protein, in non-small-cell lung cancer (NSCLC) remains unknown. We explored its role in NSCLC growth. GTSE1 was detected in NSCLC tissues and cell lines using quantitative real-time polymerase chain reaction. The clinical significance of GTSE1 levels was evaluated. Biological and apoptotic effects of GTSE1 were evaluated using transwell, cell-scratch, and MTT assays, and flow cytometry and western blotting, respectively. Its association with cellular microtubules was shown by western blotting and immunofluorescence. GTSE1 expression was upregulated in NSCLC tissues and cell lines. GTSE1 levels correlated with lymph node metastasis. Higher GTSE1 mRNA expression correlated with shorter progression-free survival. GTSE1-knockdown decreased proliferation, colony formation, invasion, and migration of NSCLC cells, and inhibited tau and stathmin-1 microtubule-associated protein expression, via the extracellular-regulated protein kinase/mitogen-activated protein kinase (ERK/MAPK) signaling pathway, and microtubule disruption. GTSE1 may promote NSCLC growth by regulating tau and stathmin-1 through the ERK/MAPK signaling pathway.

摘要

G2 和 S 期表达蛋白 1（GTSE1）是一种微管定位蛋白，其在非小细胞肺癌（NSCLC）中的作用尚不清楚。我们探讨了其在 NSCLC 生长中的作用。使用实时定量聚合酶链反应检测 NSCLC 组织和细胞系中的 GTSE1。评估 GTSE1 水平的临床意义。通过 Transwell、细胞划痕、MTT 测定、流式细胞术和 Western blot 分别评估 GTSE1 的生物学和凋亡作用。Western blot 和免疫荧光显示其与细胞微管的关联。GTSE1 在 NSCLC 组织和细胞系中表达上调。GTSE1 水平与淋巴结转移相关。较高的 GTSE1 mRNA 表达与无进展生存期缩短相关。GTSE1 敲低通过细胞外调节蛋白激酶/丝裂原活化蛋白激酶（ERK/MAPK）信号通路和微管破坏，降低 NSCLC 细胞的增殖、集落形成、侵袭和迁移，并抑制微管相关蛋白 tau 和 stathmin-1 的表达。GTSE1 可能通过 ERK/MAPK 信号通路调节 tau 和 stathmin-1 促进 NSCLC 生长。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c25f/10260487/6378c71d2fb1/TCA-14-1624-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c25f/10260487/a66ec5e9110c/TCA-14-1624-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c25f/10260487/7bfe62b04a20/TCA-14-1624-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c25f/10260487/3b4ea7dd0c48/TCA-14-1624-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c25f/10260487/e500a2467681/TCA-14-1624-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c25f/10260487/810fd3dd53f7/TCA-14-1624-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c25f/10260487/6378c71d2fb1/TCA-14-1624-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c25f/10260487/a66ec5e9110c/TCA-14-1624-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c25f/10260487/7bfe62b04a20/TCA-14-1624-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c25f/10260487/3b4ea7dd0c48/TCA-14-1624-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c25f/10260487/e500a2467681/TCA-14-1624-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c25f/10260487/810fd3dd53f7/TCA-14-1624-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c25f/10260487/6378c71d2fb1/TCA-14-1624-g002.jpg

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