Instituto de Investigación de la Cadena Láctea (IdICaL) CONICET - INTA, Ruta 34 km 227, CP: 2300 Rafaela, Santa Fe, Argentina.
Instituto de Investigación de la Cadena Láctea (IdICaL) CONICET - INTA, Ruta 34 km 227, CP: 2300 Rafaela, Santa Fe, Argentina.
Comp Immunol Microbiol Infect Dis. 2023 May;96:101980. doi: 10.1016/j.cimid.2023.101980. Epub 2023 Apr 7.
The present study aimed at the molecular detection of Anaplasma spp. in different samples obtained from cattle, goats and free-living Rhipicephalus microplus ticks from Argentina. DNA of members of the Anaplasmataceae family was detected by different PCR assays. The phylogenetic analyses of the obtained partial DNA sequences of the 16 S rDNA gene resulted in the identification of two different Anaplasma spp.: (I) Anaplasma platys-like bacteria (in blood sample from cattle and pools of R. microplus larvae and (II) Candidatus Anaplasma boleense (in blood samples from goats and one pool of R. microplus larvae of R. microplus). Candidatus A. boleense was found in two provinces that belong to different biogeographic regions, which leads to the conclusion that this bacterium may be widely distributed in Argentina. Interestingly, both Anaplasma spp. were found in the same R. microplus population in Chaco province, indicating that these two strains of Anaplasma are circulating in the same tick population. The results of this work represent the first report of the circulation of A. platys-like bacteria and Ca. A. boleense in domestic ruminants and free-living R. microplus ticks in Argentina. Further studies to determine the prevalence of infection, dispersion, clinical impact, transmission routes and cross-reactivity in serological tests of both Anaplasma species are needed.
本研究旨在从阿根廷的牛、山羊和自由生活的璃眼蜱（Rhipicephalus microplus）的不同样本中检测出无形体属（Anaplasma spp.）。通过不同的 PCR 检测方法，检测出了无形体科成员的 DNA。对获得的 16S rDNA 基因部分 DNA 序列的系统发育分析，鉴定出了两种不同的无形体属：（I）平形无浆体样细菌（在牛的血液样本和璃眼蜱幼虫的混合样本中）和（II）候选无形体（在山羊的血液样本和璃眼蜱幼虫的混合样本中）。候选无形体（Ca. Anaplasma boleense）在两个属于不同生物地理区域的省份被发现，这表明这种细菌可能在阿根廷广泛分布。有趣的是，两种无形体属都在查科省的同一璃眼蜱种群中被发现，表明这两种无形体菌株在同一蜱种群中循环。这项工作的结果代表了在阿根廷的家畜和自由生活的璃眼蜱中循环的平形无浆体样细菌和候选无形体（Ca. Anaplasma boleense）的首次报道。需要进一步的研究来确定这两种无形体的感染流行率、分布、临床影响、传播途径和血清学检测的交叉反应性。
