Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Sep 5;297:122714. doi: 10.1016/j.saa.2023.122714. Epub 2023 Apr 6.
In the present work, sulfide-doped carbon dots (S-CDs)/cadmium sulfide quantum dots (CdS QDs) ratiometric fluorescent nanosensor has been developed for sensitive and selective determination of glibenclamide (GLC) in biological fluids. The method was based on the quenching effect of GLC on the dual-emission intensity of the S-CDs/CdS QDs system at 420 nm and 650 nm, which are related to S-CDs and CdS QDs, respectively. The fluorimetric data analysis indicated that the fluorescence signals of the system were quenched by adding GLC in a concentration-dependent manner. A good linear relationship was observed between GLC concentration and the quenched fluorescence intensity of the S-CDs/CdS QDs in the range of 0.3 nM-10.0 μM. The limit of detection (LOD) value was estimated to be 0.12 nM. Furthermore, under optimum conditions, GLC was detected in spiked human serum sample (as real media) using the developed ratiometric nanosensor with an accuracy of 99.6%. According to the results, the developed dual-emission system can be used as a reliable method for the quantitative detection of GLC in biological samples.
在本工作中，开发了一种硫化物掺杂碳点（S-CDs）/硫化镉量子点（CdS QDs）比率荧光纳米传感器，用于灵敏和选择性测定生物流体中的格列本脲（GLC）。该方法基于 GLC 对 S-CDs/CdS QDs 体系在 420nm 和 650nm 处的双发射强度的猝灭效应，分别与 S-CDs 和 CdS QDs 有关。荧光数据分析表明，加入 GLC 后，该体系的荧光信号呈浓度依赖性猝灭。在 0.3nM-10.0μM 范围内，GLC 浓度与 S-CDs/CdS QDs 的猝灭荧光强度之间呈现良好的线性关系。检测限（LOD）值估计为 0.12nM。此外，在最佳条件下，使用开发的比率纳米传感器在人血清样品（实际介质）中测定 GLC 的加标回收率为 99.6%。根据结果，所开发的双发射系统可作为一种用于生物样品中 GLC 定量检测的可靠方法。
