Abdel-Gaber Rewaida, Hawsah Maysar Abu, Al-Shaebi Esam M, Al-Otaibi Tahani, Thagfan Felwa A, Al-Quraishy Saleh, Dkhil Mohamed A
Department of Zoology, College of Sciences, King Saud University, Riyadh, Saudi Arabia.
Department of Science and Technology, Al-Nairiyah University College, University of Hafr Al-Batin, Hafr Al-Batin, Saudi Arabia.
Microsc Res Tech. 2023 Jun;86(6):714-724. doi: 10.1002/jemt.24331. Epub 2023 Apr 21.
Coccidiosis is a protozoan parasitic disease affecting different animal species. Resistance has been reported for all available anticoccidial drugs. Recently, green synthesis of nanoparticles is considered a new therapeutic tool against this parasitic disease. The present work aimed to study the effect of biosynthesized nanoselenium from Azadirachta indica leaf extracts (BNS) against Eimeria papillata-induced infection in mice. The phytochemical analysis of leaf extracts contained 33 phytochemical components. The BNS was spherical with ⁓68.12 nm in diameter and an absorption peak at 308 nm via UV-spectra. The data showed that mice infected with E. papillata revealed the highest oocyst output on the 5th-day post-infection (p.i.). Infection also induced injury and inflammation of the mice jejunum. Treatment with BNS resulted in a 97.21% suppression for the oocyst output. The treated groups with BNS showed enhancement in feed intake as compared to the infected group. Histological examinations showed a significant reduction in the intracellular developmental Eimeria stages in the jejunal tissues of infected-treated mice of about 24.86 ± 2.38 stages/10 villous crypt units. Moreover, there was a significant change in the morphometry for Eimeria stages after the treatment with BNS. Infection induced a disturbance in the level of carbohydrates and protein contents in the infected mice which enhanced after treatment with BNS. In addition, BNS counteracted the E. papillata-induced loss of the total antioxidant capacity. Collectively, BNS is considered a promising anticoccidial and antioxidant effector and could be used for the treatment of coccidiosis.
球虫病是一种影响不同动物物种的原生动物寄生虫病。已报道对所有可用的抗球虫药物均产生了耐药性。最近，纳米颗粒的绿色合成被认为是对抗这种寄生虫病的一种新的治疗工具。本研究旨在探讨印楝叶提取物生物合成的纳米硒（BNS）对小鼠乳头艾美耳球虫感染的影响。叶提取物的植物化学分析含有33种植物化学成分。BNS呈球形，直径约68.12nm，通过紫外光谱在308nm处有一个吸收峰。数据显示，感染乳头艾美耳球虫的小鼠在感染后第5天的卵囊产量最高。感染还导致小鼠空肠损伤和炎症。用BNS治疗可使卵囊产量抑制97.21%。与感染组相比，BNS治疗组的采食量有所增加。组织学检查显示，感染治疗小鼠空肠组织中艾美耳球虫细胞内发育阶段显著减少，约为24.86±2.38个阶段/10个绒毛隐窝单位。此外，用BNS治疗后，艾美耳球虫阶段的形态测量有显著变化。感染导致感染小鼠碳水化合物和蛋白质含量水平紊乱，用BNS治疗后这种紊乱加剧。此外，BNS抵消了乳头艾美耳球虫诱导的总抗氧化能力的丧失。总体而言，BNS被认为是一种有前途的抗球虫和抗氧化效应物，可用于治疗球虫病。
