Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt.
Applied Science Research Center, Applied Science Private University, Amman, Jordan.
Environ Sci Pollut Res Int. 2023 Mar;30(15):44566-44577. doi: 10.1007/s11356-023-25383-0. Epub 2023 Jan 25.
Eimeriosis, an infection with Eimeria spp. that affects poultry, causes huge economic losses. Silver nanoparticles (AgNPs) have antibacterial and antifungal properties, but their action against Eimeria infection has not yet been elucidated. This study demonstrates the action of AgNPs in the treatment of mice infected with Eimeria papillata. AgNPs were prepared from Zingiber officinale rhizomes. Phytochemical screening by gas chromatography-mass spectrometry analysis (GC-MS) was used to detect active compounds. Mice were divided into five groups: uninfected mice, uninfected mice that were administered AgNPs, untreated mice infected with 10 sporulated oocysts of E. papillata, infected mice treated with AgNPs, and infected mice treated with amprolium. Characterization of the samples showed the AgNPs to have nanoscale sizes and aspherical shape. Phytochemical screening by GC-MS demonstrated the presence of 38 phytochemical compounds in the extract of Z. officinale. Mice infected with E. papillata-sporulated oocysts were observed to have many histopathological damages in the jejuna, including a decrease in the goblet cell numbers affecting the jejunal mucosa. Additionally, an increased oocyst output was also observed. The treatment of infected mice with AgNPs resulted in the improvement of the jejunal mucosa, increase in the number of goblet cell, and decrease in the number of meronts, gamonts, and developing oocysts in the jejuna. Moreover, AgNPs also led to decreased oocyst shedding in feces. The results revealed AgNPs to have an anticoccidial effect in the jejunum of E. papillata-infected mice and, thus, could be a potential treatment for eimeriosis.
艾美耳球虫病是一种由艾美耳属球虫引起的禽类感染病，会造成巨大的经济损失。银纳米粒子（AgNPs）具有抗菌和抗真菌特性，但它们对艾美耳球虫感染的作用尚未阐明。本研究旨在展示 AgNPs 在治疗感染艾美耳属球虫的小鼠中的作用。AgNPs 是由姜根茎制备的。采用气相色谱-质谱联用分析（GC-MS）进行植物化学成分筛选，以检测活性化合物。将小鼠分为五组：未感染的小鼠、给予 AgNPs 的未感染小鼠、未用 10 个孢子化卵囊感染的感染小鼠、用 AgNPs 治疗的感染小鼠和用氨丙啉治疗的感染小鼠。对样品的特征分析表明，AgNPs 具有纳米级尺寸和非球形形状。GC-MS 的植物化学成分筛选表明，姜根茎提取物中存在 38 种植物化学成分。感染艾美耳属球虫孢子化卵囊的小鼠在空肠中观察到许多组织病理学损伤，包括杯状细胞数量减少，影响空肠黏膜。此外，还观察到卵囊排出量增加。用 AgNPs 治疗感染小鼠可改善空肠黏膜，增加杯状细胞数量，减少空肠中的裂殖子、配子和发育中的卵囊数量。此外，AgNPs 还导致粪便中卵囊排出量减少。结果表明，AgNPs 对感染艾美耳属球虫的小鼠空肠具有抗球虫作用，因此可能是一种治疗艾美耳球虫病的潜在方法。
