Shyamala M, Nambu J R, Scheller R H
Brain Res. 1986 Apr 16;371(1):49-57. doi: 10.1016/0006-8993(86)90809-7.
RNA blotting and cDNA cloning techniques were used to study expression of the egg-laying hormone (ELH) gene family in the head ganglia of Aplysia californica. All head ganglia were found to express a 1800 nucleotide (nt) mRNA homologous to the ELH gene family. The nucleotide sequence of a clone isolated from a ring ganglia cDNA library demonstrates that this message encodes the ELH precursor. Further studies demonstrate the presence of a smaller, 1500 nt, transcript which encodes the peptide A precursor. However, the level of this mRNA is at least 10-fold lower than the ELH encoding message.
