College of Life Science, Qingdao Agricultural University, No. 700, Changcheng Road, Chengyang District, Qingdao, 266109, Shandong, People's Republic of China.
Department of Neurology, Peking University People's Hospital, Beijing, 100044, People's Republic of China.
Tissue Eng Regen Med. 2023 Aug;20(5):683-694. doi: 10.1007/s13770-023-00540-1. Epub 2023 Apr 21.
Type 2 diabetes (T2D) is a common metabolic disorder. Due to insufficient insulin secretion or insulin resistance, increased blood glucose often leads to impaired wound healing in T2D patients. Our previous research showed that adipose-derived stem cells (ASCs) from normal mice and T2D mice improved the cutaneous wound healing of diabetic mice. We also found that the expression of neuropeptide Y (NPY) in T2D ASCs was significantly decreased.
In order to explore the effects of NPY on ASCs and diabetic wound healing, we investigated the effects of NPY on ASCs proliferation and growth factors expression and secretion, the effects of NPY on skin fibroblasts, and the effects of NPY combined with ASCs on T2D wound healing.
The results showed that a certain concentration of NPY could promote the proliferation and the growth factors expression and secretion of ASCs, and promote the proliferation and migration of fibroblasts. At the same time, NPY and ASCs have a synergistic effect, which can promote wound healing and decrease inflammation in T2D wounds. NPY may regulate ASCs through the ERK pathway. These results are conducive to promoting ASCs and NPY in the treatment of diabetic wounds.
NPY can promote the effect of ASCs in the treatment of diabetic wounds.
2 型糖尿病（T2D）是一种常见的代谢紊乱疾病。由于胰岛素分泌不足或胰岛素抵抗，血糖升高常导致 T2D 患者的伤口愈合受损。我们之前的研究表明，正常小鼠和 T2D 小鼠来源的脂肪干细胞（ASCs）可改善糖尿病小鼠的皮肤伤口愈合。我们还发现，T2D ASC 中神经肽 Y（NPY）的表达显著降低。
为了探讨 NPY 对 ASCs 和糖尿病伤口愈合的影响，我们研究了 NPY 对 ASCs 增殖和生长因子表达和分泌的影响、NPY 对皮肤成纤维细胞的影响，以及 NPY 与 ASCs 联合对 T2D 伤口愈合的影响。
结果表明，一定浓度的 NPY 可促进 ASCs 的增殖和生长因子的表达和分泌，并促进成纤维细胞的增殖和迁移。同时，NPY 和 ASCs 具有协同作用，可促进 T2D 伤口的愈合并减少炎症。NPY 可能通过 ERK 通路调节 ASCs。这些结果有助于促进 ASCs 和 NPY 在糖尿病伤口治疗中的应用。
NPY 可增强 ASCs 治疗糖尿病伤口的效果。
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