Scott R E, Gaucher G M
Can J Microbiol. 1986 Mar;32(3):268-72. doi: 10.1139/m86-052.
The radionuclide 54MnCl2 was used to examine the cellular manganese content of submerged cultures of Penicillium urticae NRRL 2159A. Liquid-scintillation spectroscopy allowed sensitive detection of isotopic manganese in both normally supplemented and manganese-deficient cultures. The cellular manganese content in supplemented cultures showed three distinct phases, including a period of uptake that coincided with the time of transition to antibiotic biosynthesis. Such an uptake was not seen for manganese-deficient cultures, but addition of normal quantities of unlabelled manganese to the media appeared to stimulate uptake. Preliminary characterization shows this manganese uptake is not inhibited by other metal ions, does not require metabolic energy or a protein component, but is disrupted by changes in incubation temperature. The significance of these observations is discussed in the light of recent work on the requirement for manganese for antibiotic biosynthesis in this organism.
放射性核素54MnCl2被用于检测荨麻青霉NRRL 2159A submerged培养物的细胞锰含量。液体闪烁光谱法能够灵敏地检测正常添加锰和缺锰培养物中的同位素锰。添加锰的培养物中的细胞锰含量呈现出三个不同阶段，包括一个摄取期，该摄取期与向抗生素生物合成转变的时间相吻合。缺锰培养物中未观察到这种摄取现象，但向培养基中添加正常量的未标记锰似乎会刺激摄取。初步表征表明，这种锰摄取不受其他金属离子抑制，不需要代谢能量或蛋白质成分，但会因孵育温度的变化而受到干扰。根据近期关于该生物体抗生素生物合成对锰需求的研究工作，对这些观察结果的意义进行了讨论。
