School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea; Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea; Department of Biomedical Sciences, Seoul National University Graduate School, Seoul, Republic of Korea.
J Photochem Photobiol B. 2023 Jun;243:112714. doi: 10.1016/j.jphotobiol.2023.112714. Epub 2023 Apr 15.
High-energy irradiation eliminates cancer cells by destroying their genetic components. However, there are several side effects from doing this, such as fatigue, dermatitis, and hair loss, which remain obstacles to this treatment. Here, we propose a moderate method that uses low-energy white light from a light-emitting diode (LED) to selectively inhibit cancer cell proliferation without affecting normal cells.
The association between LED irradiation and cancer cell growth arrest was evaluated based on cell proliferation, viability, and apoptotic activity. Immunofluorescence, polymerase chain reaction, and western blotting were performed in vitro and in vivo to identify the metabolism related to the inhibition of HeLa cell proliferation.
LED irradiation aggravated the defective p53 signaling pathway and induced cell growth arrest in cancer cells. Consequently, cancer cell apoptosis was induced by the increased DNA damage. Additionally, LED irradiation inhibited the proliferation of cancer cells by suppressing the MAPK pathway. Furthermore, the suppression of cancer growth by the regulation of p53 and MAPK was observed in cancer-bearing mice irradiated with LED.
Our findings suggest that LED irradiation can suppress cancer cell activity and may contribute to preventing the proliferation of cancer cells after medical surgery without causing side effects.
高能量辐射通过破坏癌细胞的遗传成分来消灭癌细胞。然而，这样做有几个副作用，如疲劳、皮炎和脱发，这些仍然是治疗的障碍。在这里，我们提出了一种温和的方法，使用发光二极管（LED）发出的低能量白光选择性地抑制癌细胞增殖，而不影响正常细胞。
根据细胞增殖、活力和凋亡活性，评估 LED 照射与癌细胞生长抑制之间的关联。在体外和体内进行免疫荧光、聚合酶链反应和蛋白质印迹，以鉴定与 HeLa 细胞增殖抑制相关的代谢。
LED 照射加重了缺陷的 p53 信号通路，并诱导癌细胞生长停滞。因此，增加的 DNA 损伤诱导了癌细胞凋亡。此外，LED 照射通过抑制 MAPK 通路抑制了癌细胞的增殖。此外，在接受 LED 照射的荷瘤小鼠中观察到通过调节 p53 和 MAPK 抑制癌症生长。
我们的研究结果表明，LED 照射可以抑制癌细胞活性，并有助于在不产生副作用的情况下防止手术后癌症细胞的增殖。
