Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, Republic of Korea.
Department of Rehabilitation Medicine, Pusan National University Yangsan Hospital, Yangsan, Republic of Korea.
J Cell Physiol. 2021 Feb;236(2):1362-1374. doi: 10.1002/jcp.29943. Epub 2020 Aug 4.
The beneficial effects of light-emitting diode (LED) irradiation have been reported in various pathologies, including cancer. However, its effect in pancreatic cancer cells remains unclear. Herein, we demonstrated that blue LED of 460 nm regulated pancreatic cancer cell proliferation and apoptosis by suppressing the expression of apoptosis-related factors, such as mutant p53 and B-cell lymphoma 2 (Bcl-2), and decreasing the expression of RAC-β serine/threonine kinase 2 (AKT2), the phosphorylation of protein kinase B (AKT), and mammalian target of rapamycin (mTOR). Blue LED irradiation also increased the levels of cleaved poly-(ADP-ribose) polymerase (PARP) and caspase-3 in pancreatic cancer cells, while it suppressed AKT2 expression and inhibited tumor growth in xenograft tumor tissues. In conclusion, blue LED irradiation suppressed pancreatic cancer cell and tumor growth by regulating AKT/mTOR signaling. Our findings indicated that blue LEDs could be used as a nonpharmacological treatment for pancreatic cancer.
发光二极管（LED）照射的有益效果已在多种疾病中得到报道，包括癌症。然而，其在胰腺癌细胞中的作用尚不清楚。在此，我们证明了 460nm 的蓝光 LED 通过抑制凋亡相关因子（如突变型 p53 和 B 细胞淋巴瘤 2（Bcl-2））的表达以及降低 RAC-β丝氨酸/苏氨酸激酶 2（AKT2）、蛋白激酶 B（AKT）和雷帕霉素靶蛋白（mTOR）的磷酸化水平，从而调控胰腺癌细胞的增殖和凋亡。蓝光 LED 照射还增加了胰腺癌细胞中裂解多聚（ADP-核糖）聚合酶（PARP）和半胱天冬酶-3 的水平，同时抑制 AKT2 的表达并抑制异种移植肿瘤组织中的肿瘤生长。总之，蓝光 LED 照射通过调节 AKT/mTOR 信号通路抑制了胰腺癌细胞和肿瘤的生长。我们的研究结果表明，蓝光 LED 可作为治疗胰腺癌的非药物治疗方法。
