Yao Zhitao, An Wei, Tuerdi Maimaitituxun, Zhao Jin
Department of Trauma and Orthopedics, the First Affiliated Hospital of Xinjiang Medical University, No. 137 South Liyushan Road, Urumqi 830054, China; Oral Disease Institute of Xinjiang Uyghur Autonomous Region, No.137 South Liyushan Road, Urumqi 830054, China.
Department of Trauma and Orthopedics, the First Affiliated Hospital of Xinjiang Medical University, No. 137 South Liyushan Road, Urumqi 830054, China; Oral Disease Institute of Xinjiang Uyghur Autonomous Region, No.137 South Liyushan Road, Urumqi 830054, China.
Transl Oncol. 2023 Jul;33:101672. doi: 10.1016/j.tranon.2023.101672. Epub 2023 Apr 20.
The low 5-year survival rate of oral squamous cell carcinoma (OSCC) suggests that new prognostic indicators need to be identified to aid the clinical management of patients.
Saliva samples from OSCC patients and healthy controls were collected for proteomic and metabolomic sequencing. Gene expressed profiling was downloaded from TCGA and GEO databases. After the differential analysis, proteins with a significant impact on the prognosis of OSCC patients were screened. Correlation analysis was performed with metabolites and core proteins were identified. Cox regression analysis was utilized to stratify OSCC samples based on core proteins. The prognostic predictive ability of the core protein was then evaluated. Differences in infiltration of immune cells between the different strata were identified.
There were 678 differentially expressed proteins (DEPs), 94 intersected DEPs among them by intersecting with differentially expressed genes in TCGA and GSE30784 dataset. Seven core proteins were identified that significantly affected OSCC patient survival and strongly correlated with differential metabolites (R > 0.8). The samples were divided into high- and low-risk groups according to median risk score. The risk score and core proteins were well prognostic factor in OSCC patients. Genes in high-risk group were enriched in Notch signaling pathway, epithelial mesenchymal transition (EMT), and angiogenesis. Core proteins were strongly associated with the immune status of OSCC patients.
The results established a 7-protein signatures with the hope of early detection and the capacity for risk assessment of OSCC patient prognosis. Further providing more potential targets for the treatment of OSCC.
口腔鳞状细胞癌（OSCC）的5年生存率较低，这表明需要确定新的预后指标以辅助患者的临床管理。
收集OSCC患者和健康对照的唾液样本进行蛋白质组学和代谢组学测序。从TCGA和GEO数据库下载基因表达谱。经过差异分析，筛选出对OSCC患者预后有显著影响的蛋白质。进行代谢物相关性分析并鉴定核心蛋白质。利用Cox回归分析根据核心蛋白质对OSCC样本进行分层。然后评估核心蛋白质的预后预测能力。确定不同分层之间免疫细胞浸润的差异。
有678个差异表达蛋白（DEP），通过与TCGA和GSE30784数据集中的差异表达基因相交，其中有94个相交的DEP。鉴定出7种核心蛋白质，它们显著影响OSCC患者的生存，并与差异代谢物强烈相关（R>0.8）。根据中位风险评分将样本分为高风险组和低风险组。风险评分和核心蛋白质是OSCC患者良好的预后因素。高风险组中的基因在Notch信号通路、上皮间质转化（EMT）和血管生成中富集。核心蛋白质与OSCC患者的免疫状态密切相关。
研究结果建立了一种7蛋白特征，有望早期检测并对OSCC患者预后进行风险评估。进一步为OSCC的治疗提供更多潜在靶点。
