Department of Joint Surgery, Xi'an Hong Hui Hospital, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi Province, China; Shaanxi University of Traditional Chinese Medicine, Xianyang, Shaanxi Province, China.
Department of Joint Surgery, Xi'an Hong Hui Hospital, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi Province, China.
Immunol Lett. 2023 Jun;258:24-34. doi: 10.1016/j.imlet.2023.04.007. Epub 2023 Apr 20.
Osteoarthritis (OA) is a degenerative joint disease characterized by the destruction of articular cartilage. Tenacissoside G is a flavonoid isolated from the dry roots of Marsdenia tenacissima (Roxb) and has been shown to have anti-inflammatory effects. However, there is no report on the protective effects of Tenacissoside G on OA.
To identify the effects and mechanism of Tenacissoside G on OA.
In vitro, primary mouse chondrocytes were induced with IL-1β to establish OA model. mRNA expression of MMP-13, MMP-3, TNF-α, IL-6 and iNOS, was detected by PCR. Protein expression of Collagen-II, MMP-13, p65, p-p65, and IκBα was detected by Western blot. Collagen-II in chondrocytes was also detected by immunofluorescence. In vivo, we established DMM OA mice model. The preventive effect of Tenacissoside G on OA was observed by micro-CT and histological analysis.
In vitro, Tenacissoside G significantly inhibited the expression of iNOS, TNF-α, IL-6, MMP-3, MMP-13 and the degradation of collagen-II, Tenacissoside G also significantly suppressed NF-κB activation in chondrocytes by IL-1β-stimulated. In vivo, we demonstrated Tenacissoside G can decrease articular cartilage damage and reduce OARSI score.
These results suggest that Tenacissoside G may serve as a potential drug for the prevention and treatment of OA.
骨关节炎（OA）是一种退行性关节疾病，其特征是关节软骨破坏。远志糖苷 G 是从远志的干根中分离得到的一种黄酮类化合物，具有抗炎作用。然而，目前尚无远志糖苷 G 对 OA 保护作用的报道。
确定远志糖苷 G 对 OA 的作用及其机制。
在体外，用白细胞介素-1β诱导原代小鼠软骨细胞建立 OA 模型。通过 PCR 检测 MMP-13、MMP-3、TNF-α、IL-6 和 iNOS 的 mRNA 表达。通过 Western blot 检测 Collagen-II、MMP-13、p65、p-p65 和 IκBα的蛋白表达。通过免疫荧光检测软骨细胞中的 Collagen-II。在体内，建立 DMM OA 小鼠模型。通过 micro-CT 和组织学分析观察 Tenacissoside G 对 OA 的预防作用。
在体外，Tenacissoside G 显著抑制 iNOS、TNF-α、IL-6、MMP-3、MMP-13 的表达和 Collagen-II 的降解，Tenacissoside G 还显著抑制了 NF-κB 在 IL-1β刺激的软骨细胞中的激活。在体内，我们证明 Tenacissoside G 可以减少关节软骨损伤，降低 OARSI 评分。
这些结果表明，Tenacissoside G 可能作为一种潜在的 OA 预防和治疗药物。
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