紫檀芪 A 通过调节 NF-B 和 Nrf2/HO-1 通路缓解 OA 软骨细胞中的 IL-1 诱导的炎症。
Sappanone A Alleviated IL-1-Induced Inflammation in OA Chondrocytes through Modulating the NF-B and Nrf2/HO-1 Pathways.
机构信息
Department of Orthopaedics, The First Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an, 710061 Shaanxi, China.
出版信息
Dis Markers. 2022 Sep 16;2022:2380879. doi: 10.1155/2022/2380879. eCollection 2022.
OBJECTIVE
This study was to examine the anti-inflammatory effect of sappanone A on interleukin- (IL-) 1-stimulated osteoarthritis (OA) chondrocytes.
METHODS
Chondrocytes were pretreated with sappanone A for 2 h before subsequent IL-1 stimulation. The mRNA expression levels of iNOs, COX-2, aggrecan, and collagen-II were measured with qRT-PCR. The levels of TNF-, IL-6, IL-8, MMP-3, and MMP-13 were determined by ELISA. The protein levels of iNOs, COX-2, ADAMTS-4, ADAMTS-5, aggrecan, collagen-II, p-p65, p65, IB, Nrf2, and HO-1 were assessed by Western blot.
RESULTS
Sappanone A inhibited the IL-1-stimulated production of NO, PGE2, iNOS, COX-2, TNF-, IL-6, and IL-8 in OA chondrocytes. In addition, sappanone A suppressed the expression of MMP-3, MMP-13, ADAMTS-4, and ADAMTS-5 in IL-1-stimulated OA chondrocytes. The degradation of ECM components was reversed by sappanone A. Sappanone A prevented NF-B activation while enhanced Nrf2/HO-1 activation in IL-1-treated chondrocytes.
CONCLUSION
Sappanone A may be a potent therapeutic agent for OA.
目的
本研究旨在探讨紫檀芪 A 对白细胞介素-（IL-）1 刺激的骨关节炎（OA）软骨细胞的抗炎作用。
方法
在随后的 IL-1 刺激前，用紫檀芪 A 预处理软骨细胞 2 h。用 qRT-PCR 测定 iNOS、COX-2、聚集蛋白聚糖和胶原-II 的 mRNA 表达水平。通过 ELISA 测定 TNF-、IL-6、IL-8、MMP-3 和 MMP-13 的水平。用 Western blot 评估 iNOS、COX-2、ADAMTS-4、ADAMTS-5、聚集蛋白聚糖、胶原-II、p-p65、p65、IB、Nrf2 和 HO-1 的蛋白水平。
结果
紫檀芪 A 抑制了 OA 软骨细胞中 IL-1 刺激的 NO、PGE2、iNOS、COX-2、TNF-、IL-6 和 IL-8 的产生。此外，紫檀芪 A 抑制了 IL-1 刺激的 OA 软骨细胞中 MMP-3、MMP-13、ADAMTS-4 和 ADAMTS-5 的表达。紫檀芪 A 逆转了 ECM 成分的降解。紫檀芪 A 阻止了 NF-B 的激活，同时增强了 IL-1 处理的软骨细胞中 Nrf2/HO-1 的激活。
结论
紫檀芪 A 可能是 OA 的一种有效治疗药物。
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