Hai-De Wang, Shuai Liu, Bing-Bing Wang, Jie Liu, Jian-Zhong Xu, Wei-Guo Zhang
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, WuXi, People's Republic of China.
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, WuXi, People's Republic of China.
Adv Appl Microbiol. 2023;122:127-150. doi: 10.1016/bs.aambs.2022.11.002. Epub 2022 Dec 20.
As a semi-essential amino acid, l-arginine (l-Arg) plays an important role in food, health care, and medical treatment. At present, the main method of producing l-Arg is the use of microbial fermentation. Therefore, the selection and breeding of high-efficiency microbial strains is the top priority. To continuously improve the l-Arg production performance of the strains, a series of metabolic engineering strategies have been tried to transform the strains. The production of l-Arg by metabolically engineered Corynebacterium glutamicum (C. glutamicum) reached a relatively high level. Escherichia coli (E. coli), as a strain with great potential for l-Arg production, also has a large number of research strategies aimed at screening effective E. coli for producing l-Arg. E. coli also has a number of advantages over C. glutamicum in producing l-Arg. Therefore, it is of great significance to screen out excellent and stable E. coli to produce l-Arg. Here, based on recent research results, we review the metabolic pathways of l-Arg production in E. coli, the research progress of l-Arg production in E. coli, and various regulatory strategies implemented in E. coli.
作为一种半必需氨基酸，L-精氨酸（L-Arg）在食品、保健和医疗领域发挥着重要作用。目前，生产L-Arg的主要方法是利用微生物发酵。因此，高效微生物菌株的选育是重中之重。为了不断提高菌株的L-Arg生产性能，人们尝试了一系列代谢工程策略来改造菌株。通过代谢工程改造的谷氨酸棒杆菌（C. glutamicum）生产L-Arg已达到较高水平。大肠杆菌（E. coli）作为具有巨大L-Arg生产潜力的菌株，也有大量旨在筛选有效生产L-Arg的大肠杆菌的研究策略。大肠杆菌在生产L-Arg方面也比谷氨酸棒杆菌具有许多优势。因此，筛选出优良且稳定的生产L-Arg的大肠杆菌具有重要意义。在此，基于近期的研究成果，我们综述了大肠杆菌中L-Arg的生产代谢途径、大肠杆菌生产L-Arg的研究进展以及在大肠杆菌中实施的各种调控策略。
