Ahmann F R, Woo L, Hendrix M, Trent J M
Cancer Res. 1986 Jul;46(7):3560-4.
Thirty-one bone marrow aspirations were performed on patients with prostatic carcinoma metastatic to bone. After separation over a Ficoll-Hypaque gradient viable nucleated cells were cultured in semisolid agar. Colony formation occurred in 14 of 27 (52%) nonbacterially contaminated cultures. Characterization of cells from the colonies showed them to be consistent with malignant prostate cells. After staining, these cells were periodic acid-Schiff positive, prostatic acid phosphatase positive, and prostatic specific antigen positive. Other studies demonstrated the cells to be karyotypically abnormal, ultrastructurally similar to epithelial cells, and capable of secondary colony formation. Three bone marrow aspirate specimens did not have metastatic prostatic carcinoma detected by standard methods but did demonstrate colony formation. However, colony formation was most frequently seen when a radionuclide scan was positive at the aspiration site and when tumor cells were microscopically detectable by Wright staining of a smeared aspirate. The potential utility of colony forming cultures in prostate cancer is discussed. In working with bone marrow aspirates, additional cell separation procedures may be required to calculate and maximize plating efficiencies.
对前列腺癌骨转移患者进行了31次骨髓穿刺。在Ficoll-Hypaque梯度上分离后，将有活力的有核细胞培养于半固体琼脂中。27份未受细菌污染的培养物中有14份（52%）形成了集落。对集落中的细胞进行鉴定，结果显示它们与恶性前列腺细胞一致。染色后，这些细胞呈过碘酸希夫染色阳性、前列腺酸性磷酸酶阳性和前列腺特异性抗原阳性。其他研究表明，这些细胞核型异常，超微结构类似于上皮细胞，并且能够形成次级集落。3份骨髓穿刺标本通过标准方法未检测到转移性前列腺癌，但确实显示有集落形成。然而，当穿刺部位的放射性核素扫描呈阳性以及通过涂片穿刺物的瑞氏染色在显微镜下可检测到肿瘤细胞时，最常出现集落形成。本文讨论了集落形成培养在前列腺癌中的潜在应用价值。在处理骨髓穿刺物时，可能需要额外的细胞分离程序来计算并最大化接种效率。
