番茄金色花叶病毒A组分DNA在转基因植物中自主复制。

Tomato golden mosaic virus A component DNA replicates autonomously in transgenic plants.

作者信息

Rogers S G, Bisaro D M, Horsch R B, Fraley R T, Hoffmann N L, Brand L, Elmer J S, Lloyd A M

出版信息

Cell. 1986 May 23;45(4):593-600. doi: 10.1016/0092-8674(86)90291-6.

Abstract

Phenotypically normal petunia plants carrying chromosomal inserts of either the tomato golden mosaic virus (TGMV) A or the B component DNA, as single or tandem inserts, were obtained using an Agrobacterium tumefaciens Ti plasmid-based transformation system. Southern hybridization analysis revealed that the tandem, direct-repeat A plants contained free single and double stranded A component DNAs. No free B component DNA was detected in plants carrying tandem repeats of the B component. Progeny of self-fertilized plants appeared normal. In contrast, one-quarter of the progeny from tandem A by tandem B plant crosses showed chlorotic lesions on their leaves similar to virus symptoms. The significance of these results and the use of this method for the study of virus functions involved in TGMV replication and symptom production are discussed.

摘要

利用基于根癌土壤杆菌Ti质粒的转化系统，获得了携带番茄金色花叶病毒（TGMV）A或B组分DNA染色体插入片段的表型正常的矮牵牛植株，这些插入片段为单拷贝或串联拷贝。Southern杂交分析表明，串联的直接重复A植株含有游离的单链和双链A组分DNA。在携带B组分串联重复的植株中未检测到游离的B组分DNA。自花授粉植株的后代看起来正常。相比之下，串联A植株与串联B植株杂交产生的后代中有四分之一的叶片出现类似于病毒症状的褪绿斑。讨论了这些结果的意义以及该方法在研究TGMV复制和症状产生所涉及的病毒功能方面的应用。