通过液相色谱法和二氯酚靛酚比色法测定血浆中的抗坏血酸盐。

Ascorbate in plasma as measured by liquid chromatography and by dichlorophenolindophenol colorimetry.

作者信息

VanderJagt D J, Garry P J, Hunt W C

出版信息

Clin Chem. 1986 Jun;32(6):1004-6.

Abstract

Ascorbic acid was measured in 125 plasma samples by an automated colorimetric method involving dichlorophenolindophenol and by a "high-performance" liquid-chromatographic procedure with electrochemical detection. The two methods gave comparable results for samples with ascorbate concentrations of 1 to 20 mg/L (r = 0.97). We also measured the amount of total ascorbate (ascorbic acid + dehydroascorbic acid) in the same samples by a liquid-chromatographic procedure with precolumn derivitization of ascorbic acid. We confirmed that plasma contains little dehydroascorbic acid.

摘要

采用涉及二氯酚靛酚的自动比色法和带电化学检测的“高效”液相色谱法，对125份血浆样本中的抗坏血酸进行了测定。对于抗坏血酸盐浓度为1至20mg/L的样本，这两种方法得出了可比的结果（r = 0.97）。我们还通过对抗坏血酸进行柱前衍生的液相色谱法，测定了相同样本中总抗坏血酸（抗坏血酸+脱氢抗坏血酸）的含量。我们证实血浆中脱氢抗坏血酸含量很少。