Kone B C, Racusen L C, Whelton A, Solez K
Clin Nephrol. 1986;25 Suppl 1:S171-4.
To evaluate the combined effects of a brief ischemic insult and cyclosporine, four groups of male Munich Wistar rats were given: a) parenteral cyclosporine (60 mg/kg i.p.) for 4 days following 20 minutes of bilateral renal ischemia, b) the castor oil cyclosporine vehicle in a comparable volume and the same ischemic insult, c) saline in the same volume and ischemia, or d) saline and sham surgery. The cyclosporine animals ate and drank poorly, and therefore the other groups were pair-fed and watered with them. The cyclosporine-ischemia group developed significant renal failure. The other groups exhibited only a mild rise in blood urea nitrogen. Tubular vacuolization was a prominent feature in the cyclosporine and vehicle groups, but not in the saline groups. Vacuolization was correlated with severity of renal impairment. Lipid stains showed that many of the vacuoles contained lipid. Eosinophilic cytoplasmic inclusions were seen only in the cyclosporine or vehicle- (castor oil) treated animals. These findings emphasize the probable functional importance of tubular lesions in cyclosporine-induced acute renal failure, and suggest that the castor oil vehicle of parenteral cyclosporine may have renal effects of its own.
为评估短暂性缺血损伤与环孢素的联合作用，将四组雄性慕尼黑Wistar大鼠进行如下处理：a）双侧肾脏缺血20分钟后，腹腔注射环孢素（60毫克/千克），持续4天；b）注射蓖麻油环孢素溶媒，体积与a组相当，缺血损伤情况相同；c）注射相同体积的生理盐水并进行缺血处理；d）注射生理盐水并进行假手术。环孢素组动物进食和饮水不佳，因此对其他组动物按其进食和饮水情况进行配对饲养和供水。环孢素-缺血组出现了严重的肾衰竭。其他组动物的血尿素氮仅轻度升高。肾小管空泡化是环孢素组和溶媒组的一个显著特征，但生理盐水组未出现。空泡化与肾功能损害的严重程度相关。脂质染色显示许多空泡含有脂质。嗜酸性细胞质内含物仅在接受环孢素或溶媒（蓖麻油）处理的动物中可见。这些发现强调了肾小管病变在环孢素诱导的急性肾衰竭中可能具有的功能重要性，并提示腹腔注射环孢素的蓖麻油溶媒可能自身具有肾脏效应。
