Hexter Melissa, van Batenburg-Sherwood Joseph, Hashemi Parastoo
Department of Bioengineering, Imperial College London, SW7 2AZLondon, U.K.
ACS Meas Sci Au. 2023 Jan 11;3(2):120-126. doi: 10.1021/acsmeasuresciau.2c00059. eCollection 2023 Apr 19.
Fast scan cyclic voltammetry (FSCV) at carbon fiber microelectrodes (CFMs) is a method traditionally used for real-time quantification of neurotransmitters in biological systems. Reliable calibration of CFMs is essential for converting FSCV signals to analyte concentrations and generally employs flow injection analysis (FIA) performed with flow cells fabricated in-house. Such FSCV FIA cells often require significant and ongoing troubleshooting with pulsing, leaking, flow inconsistencies and dead volume being major causes of common challenges. In this work, we address these issues by creating a robust, plug-and-play FSCV flow cell. This novel design permits reproducible, high-precision, and stable flow injection profiles using low-cost materials to improve FSCV calibration. The ready-to-print computer-aided designs and hardware list are provided.
碳纤维微电极（CFM）上的快速扫描循环伏安法（FSCV）是一种传统上用于生物系统中神经递质实时定量的方法。CFM的可靠校准对于将FSCV信号转换为分析物浓度至关重要，通常采用内部制造的流通池进行流动注射分析（FIA）。这种FSCV FIA流通池经常需要大量且持续的故障排除，脉冲、泄漏、流量不一致和死体积是常见挑战的主要原因。在这项工作中，我们通过创建一个坚固的即插即用FSCV流通池来解决这些问题。这种新颖的设计允许使用低成本材料实现可重复、高精度和稳定的流动注射曲线，以改进FSCV校准。文中提供了可直接打印的计算机辅助设计和硬件清单。
