Whole-genome sequencing-based characterization of sp. 6(4): focus on natural product.

We have sequenced the whole genome of sp. 6(4) isolated from tomato roots that presents antifungal activity against phytopathogenic fungi, mainly . The genome has almost 7 Mb and 3368 hypothetical proteins that were analysed and characterized in Uniprot with the emphasis on biological compounds. Multilocus sequence typing (MLST) analyses were performed in an effort to characterize and identify this isolate, resulting in a new sequence type (ST), classified as ST64. Phenetic and phylogenetic trees were constructed to investigate sp. 6(4) evolution and sequence similarity, and the isolate is a strain closer to and . It is known that the genus possess huge metabolic capacity with the presence of cryptic genes. These genes are usually present in clusters, which are responsible for the production of diverse natural products, mainly antibiotics. In addition, 6(4) showed 11 biosynthetic gene clusters through antiSMASH, including 3 polyketide synthase (PKS) and non-ribosomal peptide synthase (NRPS) type clusters.