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经乳球菌（Lactococcus lactis）菌株 Plasma 刺激的浆细胞样树突状细胞可产生可溶性因子以抑制 SARS-CoV-2 复制。

Plasmacytoid dendritic cells stimulated with Lactococcus lactis strain Plasma produce soluble factors to suppress SARS-CoV-2 replication.

机构信息

AIDS Research Center, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan.

Kirin Central Research Institute, Kirin Holdings, Co., Ltd., 2-26-1 Muraoka-Higashi, Fujisawa, Kanagawa, 251-8555, Japan.

出版信息

Biochem Biophys Res Commun. 2023 Jun 25;662:26-30. doi: 10.1016/j.bbrc.2023.04.046. Epub 2023 Apr 17.

DOI:10.1016/j.bbrc.2023.04.046
PMID:37094430
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10110276/
Abstract

Innate immune responses are important in the control of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication. We have previously found a lactic acid bacteria species, Lactococcus lactis strain Plasma (LC-Plasma), which possesses specific feature to activate plasmacytoid dendritic cells (pDCs) and thus may affect innate immune responses. Here, we investigated the impact of pDC activation by LC-Plasma on SARS-CoV-2 replication in vitro. Addition of the culture supernatant of pDCs stimulated with LC-Plasma resulted in suppression of SARS-CoV-2 replication in Vero and Calu-3 cells. We confirmed interferon-α (IFN-α) secretion in the supernatant of pDCs stimulated with LC-Plasma and induction of IFN-stimulated genes in cells treated with the pDC supernatant. Anti-IFN-α antibody impaired the suppression of SARS-CoV-2 replication by the supernatant of LC-Plasma-stimulated pDCs, suggesting that IFN-α plays an important role in the SARS-CoV-2 suppression. Our results indicate the potential of LC-Plasma to induce inhibitory responses against SARS-CoV-2 replication through pDC stimulation with IFN-α secretion.

摘要

先天免疫反应在控制严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）复制中具有重要作用。我们之前发现了一种乳酸菌物种，乳球菌乳亚种 Plasma（LC-Plasma），它具有激活浆细胞样树突状细胞（pDCs）的特定特征，因此可能影响先天免疫反应。在这里，我们研究了 LC-Plasma 激活 pDCs 对 SARS-CoV-2 在体外复制的影响。添加经 LC-Plasma 刺激的 pDCs 的培养上清液导致 Vero 和 Calu-3 细胞中 SARS-CoV-2 复制受到抑制。我们证实了 LC-Plasma 刺激的 pDCs 上清液中干扰素-α（IFN-α）的分泌，并诱导了用 pDC 上清液处理的细胞中的 IFN 刺激基因。抗 IFN-α 抗体削弱了由 LC-Plasma 刺激的 pDCs 的上清液对 SARS-CoV-2 复制的抑制作用，表明 IFN-α 在 SARS-CoV-2 抑制中发挥重要作用。我们的研究结果表明，LC-Plasma 通过诱导 IFN-α 分泌刺激 pDCs，从而对 SARS-CoV-2 复制产生抑制作用的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195c/10110276/975db4f3a989/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195c/10110276/3b4818c77633/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195c/10110276/6306bee47ce5/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195c/10110276/f71cb4d95800/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195c/10110276/975db4f3a989/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195c/10110276/3b4818c77633/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195c/10110276/6306bee47ce5/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195c/10110276/f71cb4d95800/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195c/10110276/975db4f3a989/gr4_lrg.jpg

相似文献

[1]
Plasmacytoid dendritic cells stimulated with Lactococcus lactis strain Plasma produce soluble factors to suppress SARS-CoV-2 replication.

Biochem Biophys Res Commun. 2023-6-25

[2]
Plasmacytoid dendritic cell dysfunction caused by heat stress is improved by administration of strain plasma in mice.

Biosci Biotechnol Biochem. 2019-11

[3]
Induction of anti-viral genes mediated by humoral factors upon stimulation with Lactococcus lactis strain plasma results in repression of dengue virus replication in vitro.

Antiviral Res. 2018-10-28

[4]
Spherical lactic acid bacteria activate plasmacytoid dendritic cells immunomodulatory function via TLR9-dependent crosstalk with myeloid dendritic cells.

PLoS One. 2012-4-10

[5]
Plasmacytoid dendritic cells produce type I interferon and reduce viral replication in airway epithelial cells after SARS-CoV-2 infection.

bioRxiv. 2021-5-13

[6]
Immunomodulatory effect of Lactococcus lactis JCM5805 on human plasmacytoid dendritic cells.

Clin Immunol. 2013-10-25

[7]
Long-term administration of pDC stimulative lactic acid bacteria, Lactococcus lactis strain Plasma, prevents immune-senescence and decelerates individual senescence.

Exp Gerontol. 2018-6-30

[8]
Efficacy of strain plasma (LC-Plasma) in easing symptoms in patients with mild COVID-19: protocol for an exploratory, multicentre, double-blinded, randomised controlled trial (PLATEAU study).

BMJ Open. 2022-9-14

[9]
Severe COVID-19 patients have impaired plasmacytoid dendritic cell-mediated control of SARS-CoV-2.

Nat Commun. 2023-2-8

[10]
Long-term administration of pDC-Stimulative Lactococcus lactis strain decelerates senescence and prolongs the lifespan of mice.

Int Immunopharmacol. 2018-3-30

引用本文的文献

[1]
Protocol for Screening Host-Targeting Antivirals (HTAs) Using Human PBMCs and pDCs.

Bio Protoc. 2025-3-5

[2]
Overview of anti-viral effects of probiotics via immune cells in pre-, mid- and post-SARS-CoV2 era.

Front Immunol. 2023

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