Biological Resources and Post-Harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan.
Biological Resources and Post-Harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan; Department of Biology, School of Science, King Mongkut's Institute of Technology Ladkrabang, Chalongkrung Road, Ladkrabang, Bangkok 10520, Thailand.
J Biosci Bioeng. 2023 Jul;136(1):1-6. doi: 10.1016/j.jbiosc.2023.03.016. Epub 2023 Apr 22.
The improper disposal of palm oil industrial waste has led to serious environmental pollution. In this study, we isolated Paenibacillus macerans strain I6, which can degrade oil palm empty fruit bunches generated by the palm oil industry in nutrient-free water, from bovine manure biocompost and sequenced its genome on PacBio RSII and Illumina NovaSeq 6000 platforms. We obtained 7.11 Mbp of genomic sequences with 52.9% GC content from strain I6. Strain I6 was phylogenetically closely related to P. macerans strains DSM24746 and DSM24 and was positioned close to the head of the branch containing strains I6, DSM24746, and DSM24 in the phylogenetic tree. We used the RAST (rapid annotation using subsystem technology) server to annotate the strain I6 genome and discovered genes related to biological saccharification; 496 genes were related to carbohydrate metabolism and 306 genes were related to amino acids and derivatives. Among them were carbohydrate-active enzymes (CAZymes), including 212 glycoside hydrolases. Up to 23.6% of the oil palm empty fruit bunches was degraded by strain I6 under anaerobic and nutrient-free conditions. Evaluation of the enzymatic activity of extracellular fractions of strain I6 showed that amylase and xylanase activity was highest when xylan was the carbon source. The high enzyme activity and the diversity in the associated genes may contribute to the efficient degradation of oil palm empty fruit bunches by strain I6. Our results indicate the potential utility of P. macerans strain I6 for lignocellulosic biomass degradation.
棕榈油工业废物的不当处理导致了严重的环境污染。在本研究中，我们从牛粪生物堆肥中分离出一株能够在无营养水中降解棕榈油工业产生的油棕空果串的 Paenibacillus macerans 菌株 I6，并在 PacBio RSII 和 Illumina NovaSeq 6000 平台上对其基因组进行测序。我们从菌株 I6 获得了 7.11 Mbp 的基因组序列，GC 含量为 52.9%。菌株 I6 在系统发育上与 P. macerans 菌株 DSM24746 和 DSM24 密切相关，在系统发育树中与菌株 I6、DSM24746 和 DSM24 位于同一分支的头部附近。我们使用 RAST（使用子系统技术进行快速注释）服务器注释菌株 I6 基因组，发现与生物糖化相关的基因；496 个基因与碳水化合物代谢有关，306 个基因与氨基酸及其衍生物有关。其中包括碳水化合物活性酶（CAZymes），包括 212 种糖苷水解酶。在厌氧和无营养条件下，菌株 I6 可降解 23.6%的油棕空果串。对菌株 I6 胞外酶活性的评估表明，当木聚糖为碳源时，淀粉酶和木聚糖酶活性最高。高酶活性和相关基因的多样性可能有助于菌株 I6 对油棕空果串的高效降解。我们的研究结果表明，P. macerans 菌株 I6 具有用于木质纤维素生物质降解的潜力。
