在低离子强度下经甘油提取后多头绒泡菌内质滴无细胞模型的重新激活。
Reactivation of cell-free models of endoplasmic drops from Physarum polycephalum after glycerol extraction at low ionic strength.
作者信息
Achenbach F, Wohlfarth-Bottermann K E
出版信息
Eur J Cell Biol. 1986 Apr;40(2):135-8.
PMID:3709543
Abstract
The effect of calcium ions on the reactivation of cytoplasmic actomyosin contraction in cell-free models of endoplasmic drops from Physarum polycephalum after glycerol extraction at low ionic strength depends on the duration of the extraction procedure: Ca++ prevents contraction in 20-h extracted specimens, whereas after several days of extraction this Ca++-sensitivity is lost. These results indicate an inhibitory effect of Ca++ on cytoplasmic actomyosin contraction.
摘要
在低离子强度下用甘油提取多头绒泡菌内质滴的无细胞模型中，钙离子对细胞质肌动球蛋白收缩再激活的影响取决于提取过程的持续时间：钙离子可阻止在提取20小时的标本中发生收缩，而在提取几天后，这种对钙离子的敏感性就会丧失。这些结果表明钙离子对细胞质肌动球蛋白收缩具有抑制作用。
相似文献
1
Reactivation of cell-free models of endoplasmic drops from Physarum polycephalum after glycerol extraction at low ionic strength.
Eur J Cell Biol. 1986 Apr;40(2):135-8.
2
3
Reactivation of cell-free models of Physarum plasmodia after myosin reconstitution.
Eur J Cell Biol. 1988 Oct;47(1):7-13.
4
Reaction of the contractile apparatus in Physarum to injected Ca++, ATP, ADP and 5'AMP.
Cytobiologie. 1978 Oct;18(1):76-94.
5
Reactivation of cytoplasmic actomyosin in Physarum plasmodia extracted with glycerol and dimethylsulphoxide.
J Cell Sci. 1987 Mar;87 ( Pt 2):231-9. doi: 10.1242/jcs.87.2.231.
6
Mechanics of cytogels I: oscillations in physarum.
Cell Motil. 1984;4(6):469-503. doi: 10.1002/cm.970040606.
7
Reactivation of NBD-phallacidin-labelled actomyosin fibrils in cryosections of Physarum polycephalum: a new cell-free model.
Cell Biol Int Rep. 1984 Dec;8(12):1065-8. doi: 10.1016/0309-1651(84)90093-6.
8
Influence of ionic strength on the actomyosin reaction steps in contracting skeletal muscle fibers.
Biophys J. 2000 Jun;78(6):3138-49. doi: 10.1016/S0006-3495(00)76850-0.
9
Enrichment of fibrillar cytoplasmic actomyosin in protoplasmic strands of Physarum polycephalum for the production of cell-free models.
Cell Tissue Res. 1985;239(2):365-74. doi: 10.1007/BF00218017.
10
On the location of myosin in the myxomycete Physarum polycephalum and its possible function in cytoplasmic streaming.
J Mechanochem Cell Motil. 1972 Aug;1(3):125-37.
引用本文的文献
1
Calcium inhibition as an intracellular signal for actin-myosin interaction.
Proc Jpn Acad Ser B Phys Biol Sci. 2016;92(10):478-498. doi: 10.2183/pjab.92.478.
2
Dynamic aspects of the contractile system in Physarum plasmodium. III. Cyclic contraction-relaxation of the plasmodial fragment in accordance with the generation-degeneration of cytoplasmic actomyosin fibrils.
J Cell Biol. 1987 Jul;105(1):381-6. doi: 10.1083/jcb.105.1.381.
3
Regulation by Ca(2+)-calmodulin of the actin-bundling activity of Physarum 210-kDa protein.
J Muscle Res Cell Motil. 1992 Jun;13(3):321-8. doi: 10.1007/BF01766460.
Zotero 插件