Effects of increased cell mass and altered gene dosage on the timing of initiation of macronuclear DNA synthesis in Paramecium tetraurelia. Implications for cell cycle regulation.

In Paramecium, cell mass and macronuclear DNA content can vary substantially, and both variables affect the timing of initiation of macronuclear DNA synthesis. Cells normally begin macronuclear DNA synthesis at 0.25 in the cell cycle when the mean cell mass is about 119% of the initial value. Gene mutations were used to alter cell size by temporarily blocking cell cycle progression and to change DNA content by altering the segregation pattern of macronuclear material to daughter nuclei at fission. Changes in cell mass or macronuclear DNA content imposed at fission or in the subsequent G1 interval do not affect the timing of initiation of DNA synthesis in that cell cycle, but do affect the timing of initiation of DNA synthesis in the subsequent cell cycle. The progeny of cells with lower than average macronuclear DNA content tend to initiate DNA synthesis earlier than other cells. The G1 interval is proportionally shortened when initial cell mass is greater than normal, and no measurable G1 interval is present when initial cell mass equals or exceeds the normal cell mass present at initiation of DNA synthesis. These results suggest that the timing of initiation of DNA synthesis is established during the preceding cell cycle and that the 'timer' mechanism is not significantly affected by either drastic changes in gene dosage or gene concentration during the G1 interval.