Fernandez Y, Boigegrain R A, Cambon-Gros C, Deltour P, Mitjavila S
FEBS Lett. 1986 May 26;201(1):119-23. doi: 10.1016/0014-5793(86)80582-8.
The importance of the hydrophobic effect of exogenous substances and of modifications of membrane order on D-glucose uptake are still poorly defined. Our results show that the concentrative Na+ -coupled D-glucose uptake of rat enterocyte brush border membrane vesicles is inhibited by N-phenylcarbamates increase the membrane order. However, since the concentrations required for membrane order increase are much greater than those active on D-glucose uptake, the effects on lipid order cannot be responsible for the inhibition of D-glucose uptake. Measurements of D-glucose uptake under conditions of Na+ equilibrium show that these carbamates do not act directly on the carrier but indirectly by favouring the dissipation of the Na+ gradient.
外源性物质的疏水作用以及膜有序性的改变对D - 葡萄糖摄取的重要性仍未明确界定。我们的结果表明，大鼠肠上皮细胞刷状缘膜囊泡的钠耦联D - 葡萄糖浓缩摄取受到N - 苯基氨基甲酸酯类的抑制，这类物质会增加膜的有序性。然而，由于增加膜有序性所需的浓度远高于对D - 葡萄糖摄取有活性的浓度，所以对脂质有序性的影响并非是D - 葡萄糖摄取受抑制的原因。在钠平衡条件下对D - 葡萄糖摄取的测量表明，这些氨基甲酸酯类并非直接作用于载体，而是通过促进钠梯度的耗散间接发挥作用。
