Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Zoonoses Surveillance Division, Health Surveillance Coordination, Municipal Health Department, São Paulo, Brazil; One Health Brazilian Resistance Project (OneBR), Brazil.
One Health Brazilian Resistance Project (OneBR), Brazil; Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil.
J Glob Antimicrob Resist. 2023 Jun;33:256-259. doi: 10.1016/j.jgar.2023.04.013. Epub 2023 Apr 23.
The aim of this study was to perform a genomic investigation of a multiple fluoroquinolone-resistant Leclercia adecarboxylata strain isolated from a synanthropic pigeon in São Paulo, Brazil.
Whole-genome sequencing was performed using an Illumina platform, and in silico deep analyses of the resistome were performed. Comparative phylogenomics was conducted using a global collection of publicly available genomes of L. adecarboxylata strains isolated from human and animal hosts.
L. adecarboxylata strain P62P1 displayed resistance to human (norfloxacin, ofloxacin, ciprofloxacin, and levofloxacin) and veterinary (enrofloxacin) fluoroquinolones. This multiple quinolone-resistant profile was associated with mutations in the gyrA (S83I) and parC (S80I) genes and the presence of the qnrS gene within an ISKpn19-orf-qnrS1-ΔIS3-bla module, previously identified in L. adecarboxylata strains isolated from pig feed and faeces in China. Genes associated with arsenic, silver, copper, and mercury resistance were also predicted. Phylogenomic analysis revealed clustering (378-496 single nucleotide polymorphism differences) with two L. adecarboxylata strains isolated from human and fish sources in China and Portugal, respectively.
L. adecarboxylata is a Gram-negative bacterium of the Enterobacterales order and is considered an emergent opportunistic pathogen. Since L. adecarboxylata has adapted to human and animal hosts, genomic surveillance is highly recommended, in order to identify the emergence and spread of resistant lineages and high-risk clones. In this regard, this study provides genomic data that can help clarify the role of synanthropic animals in the dissemination of clinically relevant L. adecarboxylata within a One Health context.
本研究旨在对从巴西圣保罗一只共生鸽子中分离出的多重氟喹诺酮耐药性产酸克雷伯菌进行基因组研究。
使用 Illumina 平台进行全基因组测序，并对耐药组进行深入的计算机分析。使用从人类和动物宿主中分离的产酸克雷伯菌全球公开基因组数据集进行比较系统基因组学分析。
产酸克雷伯菌 P62P1 株对人类（诺氟沙星、氧氟沙星、环丙沙星和左氧氟沙星）和兽医（恩诺沙星）氟喹诺酮类药物均具有耐药性。这种多重喹诺酮类耐药表型与 gyrA（S83I）和 parC（S80I）基因突变以及 qnrS 基因的存在有关，该基因位于 ISKpn19-orf-qnrS1-ΔIS3-bla 模块内，先前在中国分离自猪饲料和粪便的产酸克雷伯菌中已发现该模块。还预测了与砷、银、铜和汞耐药相关的基因。系统基因组分析显示，与分别从中国人类和鱼类来源中分离出的两株产酸克雷伯菌聚类（378-496 个单核苷酸多态性差异）。
产酸克雷伯菌是肠杆菌目下的革兰氏阴性细菌，被认为是一种新兴的机会性病原体。由于产酸克雷伯菌已适应人类和动物宿主，强烈建议进行基因组监测，以识别耐药谱系和高风险克隆的出现和传播。在这方面，本研究提供了基因组数据，可以帮助阐明共生动物在 One Health 背景下传播临床相关产酸克雷伯菌的作用。
