Virology Laboratory, Graduate Program in Microbial Biology, Brazil.
Laboratory of Environmental Microbiology, Graduate Program in Microbial Biology, Brazil.
Microb Pathog. 2023 Jul;180:106119. doi: 10.1016/j.micpath.2023.106119. Epub 2023 Apr 23.
Streptococcus pneumoniae is a bacterium that causes serious infections, including pneumonia. The limited range of available vaccines and the rise of antibiotic-resistant bacteria mean that new treatments are needed. This study looked at the potential of quercetin as an antimicrobial agent against S. pneumoniae in both isolation and in biofilms. The researchers used microdilution tests, checkerboard assays, and death curve assays, as well as in silico and in vitro cytotoxicity evaluations. They found that quercetin at a concentration of 125.0 μg/mL had both inhibitory and bactericidal effects against S. pneumoniae, and these effects were increased when quercetin was combined with ampicillin. Quercetin also reduced the growth of pneumococcal biofilms. In addition, quercetin (absence or in combination with ampicillin) reduced the death time of Tenebrio molitor larvae compared to the infection control. The study also demonstrated that quercetin had low toxicity in both in silico and in vivo assays, suggesting that it could be a promising treatment for infections caused by S. pneumoniae.
肺炎链球菌是一种可导致严重感染的细菌，包括肺炎。可用疫苗种类有限，且抗生素耐药菌不断增加，这意味着需要新的治疗方法。本研究着眼于槲皮素作为一种抗肺炎链球菌的抗菌剂的潜力，包括在分离状态和生物膜状态下的效果。研究人员使用微量稀释试验、棋盘试验、死亡曲线试验以及体内外细胞毒性评估。他们发现，槲皮素在浓度为 125.0μg/mL 时对肺炎链球菌具有抑制和杀菌作用，当与氨苄西林联合使用时，这些作用会增强。槲皮素还能抑制肺炎链球菌生物膜的生长。此外，与感染对照组相比，槲皮素（单独或与氨苄西林联合）能减少黄粉虫幼虫的死亡时间。该研究还表明，槲皮素在体内和体外试验中均具有低毒性，这表明它可能是治疗肺炎链球菌感染的一种有前途的方法。
