Department of Chemistry - BMC, Science for Life Laboratory, Uppsala University, Uppsala 75124, Sweden.
Centre for Translational Microbiome Research (CTMR), Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
Chem Commun (Camb). 2023 May 11;59(39):5843-5846. doi: 10.1039/d3cc01223a.
The microbiome has been identified to have a key role for the physiology of their human host. One of the major impacts is the clearance of bacterial pathogens. We have now developed a chemoselective probe methodology for the absolute quantification of short-chain fatty acids at low nM concentrations, with high reproducibility and spiked isotope labelled internal standards. Immobilization to magnetic beads allows for separation from the matrix and the tagged metabolites upon bioorthogonal cleavage can be analyzed UHPLC-MS. The major advantage of our sensitive method is the simple combination with global metabolomics analysis as only a small sample volume is required. We have applied this chemical metabolomics strategy for targeted SCFA analysis combined with global metabolomics on gut microbiome co-cultures with and investigated the effect of antibiotic treatment.
微生物组被认为对其人类宿主的生理学起着关键作用。其中一个主要影响是清除细菌病原体。我们现在已经开发出一种用于绝对定量低纳摩尔浓度下短链脂肪酸的化学选择性探针方法，具有高重现性和加标同位素标记内标。固定在磁性珠上允许在生物正交裂解后与基质分离，并且可以分析标记的代谢物 UHPLC-MS。我们灵敏方法的主要优点是可以与全局代谢组学分析简单结合，因为仅需要小体积的样品。我们已经将这种化学代谢组学策略应用于与肠道微生物组共培养物的靶向 SCFA 分析与全局代谢组学相结合，并研究了抗生素治疗的影响。
