The State Key Laboratory of Subtropical Silviculture, Bamboo Industry Institute, Zhejiang A&F University, Hangzhou, 311300 Zhejiang, China.
Tree Physiol. 2023 Aug 11;43(8):1416-1431. doi: 10.1093/treephys/tpad055.
An appropriate amount of phosphate fertilizer can improve the germination rate of bamboo buds and increase the bamboo shoot output. However, the underlying biological mechanisms of phosphate fertilizer in bamboo shoot development have not been systematically reported. Herein, the effects of low (LP, 1 μM), normal (NP, 50 μM) and high (HP, 1000 μM) phosphorus (P) on the growth and development of moso bamboo (Phyllostachys edulis) tiller buds were first investigated. Phenotypically, the seedling biomass, average number of tiller buds and bud height growth rate under the LP and HP treatments were significantly lower than those under the NP treatment. Next, the microstructure difference of tiller buds in the late development stage (S4) at three P levels was analyzed. The number of internode cells and vascular bundles were significantly lower in the LP treatments than in the NP treatments. The relative expression levels of eight P transport genes, eight hormone-related genes and four bud development genes at the tiller bud developmental stage (S2-S4) and the tiller bud re-tillering stage were analyzed with real-time polymerase chain reaction. The results showed that the expression trends for most P transport genes, hormone-related genes and bud development genes from S2 to S4 were diversified at different P levels, and the expression levels were also different at different P levels. In the tiller bud re-tillering stage, the expression levels of seven P transport genes and six hormone-related genes showed a downward trend with increasing P level. REV expression level decreased under LP and HP conditions. TB1 expression level increased under HP condition. Therefore, we conclude that P deficiency inhibits tiller bud development and re-tillering, and that P depends on the expression of REV and TB1 genes and auxin, cytokinin and strigolactones synthesis and transporter genes to mediate tiller bud development and re-tillering.
适量的磷肥可以提高竹芽的萌发率，增加竹笋产量。然而，磷肥在竹笋发育中的潜在生物学机制尚未得到系统报道。在此，我们首次研究了低磷（LP，1 μM）、正常磷（NP，50 μM）和高磷（HP，1000 μM）对毛竹（Phyllostachys edulis）分蘗芽生长发育的影响。表型上，LP 和 HP 处理下的幼苗生物量、平均分蘗芽数和芽高生长速率显著低于 NP 处理。接下来，分析了三个磷水平下分蘗芽后期（S4）的微观结构差异。LP 处理下的节间细胞和维管束数量明显低于 NP 处理。用实时聚合酶链反应分析了分蘗芽发育阶段（S2-S4）和分蘗芽再分蘗阶段的 8 个磷转运基因、8 个激素相关基因和 4 个芽发育基因的相对表达水平。结果表明，大多数磷转运基因、激素相关基因和芽发育基因从 S2 到 S4 的表达趋势在不同的磷水平下多样化，表达水平在不同的磷水平下也不同。在分蘗芽再分蘗阶段，随着磷水平的增加，7 个磷转运基因和 6 个激素相关基因的表达水平呈下降趋势。LP 和 HP 条件下 REV 的表达水平降低。HP 条件下 TB1 的表达水平增加。因此，我们得出结论，磷缺乏抑制分蘗芽的发育和再分蘗，磷依赖于 REV 和 TB1 基因的表达以及生长素、细胞分裂素和独脚金内酯的合成和转运基因来调节分蘗芽的发育和再分蘗。
