Department of Chemical and Biochemical Engineering, Technical University of Denmark, Kgs. Lyngby DK-2800, Denmark.
School of Biosystems & Food Engineering, University College Dublin, Agriculture Building, UCD Belfield, Dublin 4, Ireland.
Bioresour Technol. 2023 Jul;380:129078. doi: 10.1016/j.biortech.2023.129078. Epub 2023 Apr 24.
To substitute petroleum-based materials with bio-based alternatives, microbial fermentation combined with inexpensive biomass is suggested. In this study Saccharina latissima hydrolysate, candy-factory waste, and digestate from full-scale biogas plant were explored as substrates for lactic acid production. The lactic acid bacteria Enterococcus faecium, Lactobacillus plantarum, and Pediococcus pentosaceus were tested as starter cultures. Sugars released from seaweed hydrolysate and candy-waste were successfully utilized by the studied bacterial strains. Additionally, seaweed hydrolysate and digestate served as nutrient supplements supporting microbial fermentation. According to the highest achieved relative lactic acid production, a scaled-up co-fermentation of candy-waste and digestate was performed. Lactic acid reached a concentration of 65.65 g/L, with 61.69% relative lactic acid production, and 1.37 g/L/hour productivity. The findings indicate that lactic acid can be successfully produced from low-cost industrial residues.
建议用微生物发酵结合廉价生物质替代石油基材料。本研究以裙带菜水解物、糖果厂废料和大型沼气厂的消化物为原料，探索了用于生产乳酸的生物基替代品。选用屎肠球菌、植物乳杆菌和戊糖片球菌作为发酵起始菌。研究的细菌菌株成功利用了海藻水解物和糖果废料中释放的糖。此外，海藻水解物和消化物还可以作为营养补充剂，支持微生物发酵。根据最高相对乳酸产量，对糖果废料和消化物进行了放大规模的共发酵。乳酸浓度达到 65.65g/L，相对乳酸产量为 61.69%，产率为 1.37g/L/h。研究结果表明，可以成功地从低成本工业废物中生产乳酸。
