Technology Innovation Research Division, World Institute of Kimchi, Gwangju, Republic of Korea; Department of Agricultural Chemistry, Chonnam National University, Gwangju 61186, Republic of Korea.
Technology Innovation Research Division, World Institute of Kimchi, Gwangju, Republic of Korea.
Sci Total Environ. 2024 Feb 20;912:169521. doi: 10.1016/j.scitotenv.2023.169521. Epub 2023 Dec 22.
Lactic acid is an important industrial precursor; however, high substrate costs are a major challenge in microbial fermentation-based lactic acid production. Coffee waste is a sustainable feedstock alternative for lactic acid production via microbial fermentation. Herein, the feasibility of coffee waste as a feedstock was explored by employing appropriate pretreatment methods and optimizing enzyme combinations. Coffee waste pretreatment with hydrogen peroxide and acetic acid along with a combination of Viscozyme L, Celluclast 1.5 L, and Pectinex Ultra SP-L achieved the 78.9 % sugar conversion rate at a substrate concentration of 4 % (w/v). Lactiplantibacillus plantarum WiKim0126-induced fermentation with a 4 % solid loading yielded a lactic acid concentration of 22.8 g/L (99.6 % of the theoretical maximum yield) and productivity of 0.95 g/L/h within 24 h. These findings highlight the viability of coffee waste as an eco-friendly resource for sustainable lactic acid production.
乳酸是一种重要的工业前体，但微生物发酵法生产乳酸的主要挑战是高底物成本。咖啡废料是一种可持续的原料替代物，可通过微生物发酵生产乳酸。在此，通过采用适当的预处理方法和优化酶组合，探讨了咖啡废料作为原料的可行性。采用过氧乙酸和乙酸对咖啡废料进行预处理，并结合使用 Viscozyme L、Celluclast 1.5 L 和 Pectinex Ultra SP-L 的组合，在底物浓度为 4%（w/v）时可实现 78.9%的糖转化率。利用植乳杆菌 WiKim0126 诱导发酵，在 4%的固体负荷下，24 小时内可得到 22.8g/L 的乳酸浓度（达到理论最大产量的 99.6%）和 0.95g/L/h 的产率。这些发现表明，咖啡废料作为一种环保资源，具有可持续生产乳酸的潜力。
