体内暴露于紫外线B后大鼠晶状体中TP53 mRNA表达的方差成分估计
Estimation of the Variance Components in TP53 mRNA Expression in the Rat Lens after in vivo Exposure to Ultraviolet Radiation B.
作者信息
Galichanin Konstantin
机构信息
Gullstrand Lab, Section of Ophthalmology, Department of Surgical Sciences, Uppsala University, Uppsala, Sweden.
出版信息
Biomed Hub. 2023 Apr 21;8(1):42-45. doi: 10.1159/000530042. eCollection 2023 Jan-Dec.
INTRODUCTION
The aim of this study was to investigate the variance components in TP53 mRNA expression after in vivo exposure to double threshold dose of ultraviolet radiation B (UVR-B).
METHODS
Twelve six-week-old female albino Sprague-Dawley rats were exposed to double threshold dose (8 kJ/m) of UVR-B unilaterally and sacrificed at 1, 3, 8, and 24 h after exposure. Lenses were enucleated, and TP53 mRNA expression was detected by qRT-PCR. Variance components for groups, animals, and measurements were estimated with analysis of variance.
RESULTS
The variance for groups is 0.15 rel.. The variance for animals is 0.29 rel.. The variance for measurements is 0.32 rel..
CONCLUSION
The variance for animals is in the same order as the variance for measurements. The reduction of the variance for measurements is needed in order to obtain the acceptable level of detection of the difference in TP53 mRNA expression and the reduction in sample size.
引言
本研究旨在调查体内暴露于双阈值剂量紫外线B（UVR-B）后TP53 mRNA表达的方差成分。
方法
将12只六周龄雌性白化病斯普拉格-道利大鼠单侧暴露于双阈值剂量（8 kJ/m）的UVR-B，并在暴露后1、3、8和24小时处死。摘除晶状体，通过qRT-PCR检测TP53 mRNA表达。采用方差分析估计组、动物和测量的方差成分。
结果
组的方差为0.15 rel.。动物的方差为0.29 rel.。测量的方差为0.32 rel.。
结论
动物的方差与测量的方差处于同一量级。为了获得可接受的TP53 mRNA表达差异检测水平并减少样本量，需要降低测量的方差。
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