Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis, France.
Institut de Biologie de l'École Normale Supérieure, Paris, France.
FEBS Lett. 2023 Jun;597(12):1623-1637. doi: 10.1002/1873-3468.14630. Epub 2023 May 8.
The MIR449 genomic locus encompasses several regulators of multiciliated cell (MCC) formation (multiciliogenesis). The miR-449 homologs miR-34b/c represent additional regulators of multiciliogenesis that are transcribed from another locus. Here, we characterized the expression of BTG4, LAYN, and HOATZ, located in the MIR34B/C locus using single-cell RNA-seq and super-resolution microscopy from human, mouse, or pig multiciliogenesis models. BTG4, LAYN, and HOATZ transcripts were expressed in both precursors and mature MCCs. The Layilin/LAYN protein was absent from primary cilia, but it was expressed in apical membrane regions or throughout motile cilia. LAYN silencing altered apical actin cap formation and multiciliogenesis. HOATZ protein was detected in primary cilia or throughout motile cilia. Altogether, our data suggest that the MIR34B/C locus may gather potential actors of multiciliogenesis.
MIR449 基因组位点包含几个调控多纤毛细胞 (MCC) 形成 (多纤毛发生) 的调节因子。miR-449 的同源物 miR-34b/c 是另一个从另一个基因座转录的多纤毛发生的额外调节因子。在这里，我们使用来自人类、小鼠或猪多纤毛发生模型的单细胞 RNA-seq 和超分辨率显微镜，对位于 MIR34B/C 基因座的 BTG4、LAYN 和 HOATZ 的表达进行了表征。BTG4、LAYN 和 HOATZ 的转录本在前体细胞和成熟的 MCC 中均有表达。Layilin/LAYN 蛋白不存在于初级纤毛中，但它在顶膜区域或整个运动纤毛中表达。LAYN 沉默改变了顶端肌动蛋白帽的形成和多纤毛发生。HOATZ 蛋白在初级纤毛或整个运动纤毛中均有检测到。总的来说，我们的数据表明 MIR34B/C 基因座可能聚集了多纤毛发生的潜在作用因子。
