College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
Ningbo Institute of Zhejiang University, Ningbo 315100, China.
Food Funct. 2023 May 22;14(10):4681-4695. doi: 10.1039/d3fo00250k.
This study investigated the immunomodulatory effects of soft-shelled turtle () peptide (TP) and Chinese pond turtle () peptide (TMP) on the intestinal mucosal immune system (IMIS). The results demonstrated that TP and TMP improved holistic immunity by restoring the vital immune organ atrophy and proliferation capacity of spleen immune cells. Moreover, TP and TMP significantly increased the serum content of IgA and cytokines that are responsible for immune cell activation and antigen clearance. TP and TMP promoted intestinal B cell activation, class switching recombination, and antibody secreting processes in a T cell-independent manner to increase the SIgA content. Furthermore, TP and TMP enhanced the intestinal barrier by increasing the protein expression of tight junctions (TJs) and adhesion junctions (AJs) and ameliorating the intestinal morphology. Mechanistically, TP and TMP activated the AHR/IL-22/STAT3/IL-6 axis to enhance the IgA response and improve the intestinal barrier, indicating their potential in intestinal health modulation.
本研究探讨了软壳龟（ ）肽（TP）和中华鳖（ ）肽（TMP）对肠道黏膜免疫系统（IMIS）的免疫调节作用。结果表明，TP 和 TMP 通过恢复重要免疫器官脾免疫细胞的萎缩和增殖能力来改善整体免疫力。此外，TP 和 TMP 显著增加了 IgA 和细胞因子的血清含量，这些细胞因子负责免疫细胞的激活和抗原清除。TP 和 TMP 以 T 细胞非依赖的方式促进肠道 B 细胞的激活、类别转换重组和抗体分泌过程，从而增加 SIgA 的含量。此外，TP 和 TMP 通过增加紧密连接（TJs）和黏附连接（AJs）的蛋白表达以及改善肠道形态来增强肠道屏障。在机制上，TP 和 TMP 激活了 AHR/IL-22/STAT3/IL-6 轴，增强了 IgA 反应，改善了肠道屏障，表明它们在肠道健康调节方面具有潜力。
