Li Jinming, An Zhe, Luo Junyu, Zhu Xiangzhen, Wang Li, Zhang Kaixin, Li Dongyang, Ji Jichao, Niu Lin, Gao Xueke, Cui Jinjie
Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China.
Insects. 2023 Mar 25;14(4):314. doi: 10.3390/insects14040314.
Glover is an important agricultural pest distributed worldwide. Gahan is the main parasitoid wasp of . Previous studies have shown that parasitization causes reduced egg production in , but the effects of parasitism on the symbiotic bacteria in the host ovaries are unknown.
In this study, we analyzed the microbial communities in the ovaries of without and after parasitization. Whether parasitized or not, was the dominant genus of symbiotic bacteria in the ovaries, followed by facultative symbionts including , , and . The relative abundance of in the aphid ovary increased after parasitization for 1 d in both third-instar nymph and adult stages, but decreased after parasitization for 3 d. The shifts in the relative abundance of in both stages were the same as those observed for In addition, the relative abundance of remarkably decreased after parasitization for 1 d and increased after parasitization for 3 d. A functional predictive analysis of the control and parasitized ovary microbiomes revealed that pathways primarily enriched in parasitization were "amino acid transport and metabolism" and "energy production and conversion." Finally, RT-qPCR analysis was performed on , , and . The results of RT-qPCR were the same as the results of 16S rDNA sequencing.
These results provide a framework for investigating shifts in the microbial communities in host ovaries, which may be responsible for reduced egg production in aphids. These findings also broaden our understanding of the interactions among aphids, parasitoid wasps, and endosymbionts.
格洛弗蚜是一种分布于全球的重要农业害虫。加汉氏蚜小蜂是其主要寄生蜂。先前的研究表明，寄生会导致格洛弗蚜产卵量减少，但寄生对宿主卵巢中共生细菌的影响尚不清楚。
在本研究中，我们分析了未被寄生和被寄生的格洛弗蚜卵巢中的微生物群落。无论是否被寄生，不动杆菌属都是卵巢中共生细菌的优势属，其次是兼性共生菌，包括沙雷氏菌属、嗜麦芽窄食单胞菌属和假单胞菌属。在若虫和成虫阶段，被寄生1天后，格洛弗蚜卵巢中不动杆菌属的相对丰度均增加，但被寄生3天后则下降。两个阶段中不动杆菌属相对丰度的变化与沙雷氏菌属的变化相同。此外，被寄生1天后，嗜麦芽窄食单胞菌属的相对丰度显著下降，被寄生3天后则增加。对未被寄生和被寄生的卵巢微生物组进行功能预测分析发现，寄生后主要富集的途径是“氨基酸转运和代谢”以及“能量产生和转换”。最后，对沙雷氏菌属、嗜麦芽窄食单胞菌属和不动杆菌属进行了RT-qPCR分析。RT-qPCR结果与16S rDNA测序结果一致。
这些结果为研究宿主卵巢中微生物群落的变化提供了一个框架，这可能是导致蚜虫产卵量减少的原因。这些发现也拓宽了我们对蚜虫、寄生蜂和内共生体之间相互作用的理解。
