Souza Maria Eliza Cota E, Nóbrega Filomena, Bento Albino António
Mountain Research Center (CIMO), Polytechnic Institute of Bragança, 5300-253 Bragança, Portugal.
Associate Laboratory for Sustainability and Technology in Mountain Regions, Polytechnic Institute of Bragança, 5300-253 Bragança, Portugal.
Insects. 2023 Mar 31;14(4):342. doi: 10.3390/insects14040342.
The chestnut moth ( Hübner) and the chestnut weevil ( Gyllenhal) cause serious damage to European producers and companies that transform and market the chestnut. The objective of the present work was to evaluate, under field conditions, the possibilities of (Bals.-Criv.) Vuill. to infect and kill the larvae of the two main carpophagous pests of European chestnut, in treatments directed at the soil. For this purpose, the surfaces of vases were sprayed with two concentrations of conidia/mL 5 × 10 (T1) and 1 × 10 (T2). The control (T0) was sprayed with distilled water. Larval mortality and infection were evaluated on five dates (D8 to D220). Confirmation of the fungus present in the larva was performed by molecular analysis. The results obtained are promising for the use of as a biological control agent against these key pests of the chestnut crop. There were no significant differences in mortality between the T1 and T2 modalities, however, they were significantly higher than the control. In the case of total mortality (dead and infected larvae), no significant differences were observed for either. In the case of , the T2 modality obtained better results in terms of total mortality.
栗实蛾（许布纳）和栗实象鼻虫（吉伦哈尔）对欧洲的栗树生产者以及从事栗子加工和销售的公司造成了严重损害。本研究的目的是在田间条件下，评估在针对土壤的处理中，[具体真菌名称未给出]（巴尔 - 克里夫）·维伊尔感染并杀死欧洲栗两种主要蛀果害虫幼虫的可能性。为此，用两种分生孢子浓度/毫升（5×10（T1）和1×10（T2））对花盆表面进行喷雾处理。对照组（T0）用蒸馏水喷雾。在五个日期（D8至D220）评估幼虫死亡率和感染情况。通过分子分析确认幼虫体内存在的真菌。所获得的结果对于将[具体真菌名称未给出]用作防治栗树作物这些关键害虫的生物防治剂很有前景。T1和T2处理方式之间的死亡率没有显著差异，然而，它们显著高于对照组。就总死亡率（死亡和感染的幼虫）而言，[具体真菌名称未给出]也未观察到显著差异。对于[具体真菌名称未给出]，T2处理方式在总死亡率方面取得了更好的结果。
