Hasan Md Mahbub, Hossain Md Akhtar, Athanassiou Christos G
Department of Zoology, Rajshahi University, Rajshahi 6205, Bangladesh.
Laboratory of Entomology and Agricultural Zoology, Department of Agriculture, Crop Production and Rural Environment, University of Thessaly, Phytokou Str., 38446 Volos, Greece.
Insects. 2023 Mar 31;14(4):344. doi: 10.3390/insects14040344.
The sterile insect technique (SIT) is considered an environmentally friendly, autocidal control tactic to manage insect pests. This work dealt with the improvement of quality management of the Indian meal moth (Hübner) for enhanced efficacy of the SIT. The results indicated that egg hatching of irradiated mature eggs of was higher than that of younger eggs, indicating that mature eggs were significantly more tolerant than younger eggs. Moreover, our data revealed that a dose of 500 Gy completely prevented pupal formation in irradiated young and mature larvae. Crosses between irradiated and non-irradiated adults resulted in considerable variations in fecundity. The mating competitiveness index (CI) value was higher for a ratio of 5:1:1 (sterile male, fertile male, and fertile female, respectively) as compared with the ratio 1:1:1 for the irradiated individuals of all life stages. Low temperature (5 °C) maintenance of irradiated pupae significantly affected adult emergence. Using cylinders to assess flight ability, we found that the flight performance of adults that were developed from cold treated irradiated pupae was influenced by cylinder diameter, cylinder height and the number of hours the insects were in the cylinders. The percentage of DNA damage of the reproductive organs of adults developed from cold treated pupae that were irradiated with 100 and 150 Gy varied significantly. The results of this study should be used to implement pilot-scale field tests aiming at a sterile- to-fertile male ratio of 5 to 1.
不育昆虫技术（SIT）被认为是一种用于防治害虫的环境友好型自灭控制策略。这项工作致力于改进印度谷螟（Hübner）的质量管理，以提高不育昆虫技术的效果。结果表明，辐照成熟卵的孵化率高于未成熟卵，这表明成熟卵比未成熟卵具有显著更高的耐受性。此外，我们的数据显示，500戈瑞的剂量完全阻止了辐照后的幼龄和成熟幼虫形成蛹。辐照成虫与未辐照成虫杂交导致繁殖力出现显著差异。对于所有生命阶段的辐照个体，不育雄虫、可育雄虫和可育雌虫比例为5:1:1时的交配竞争力指数（CI）值高于比例为1:1:1时。辐照蛹在低温（5℃）下保存显著影响成虫羽化。使用圆筒评估飞行能力时，我们发现由经冷处理的辐照蛹发育而成的成虫的飞行性能受圆筒直径、圆筒高度以及昆虫在圆筒内停留的小时数影响。经100和150戈瑞辐照且经冷处理的蛹发育而成的成虫，其生殖器官的DNA损伤百分比存在显著差异。本研究结果应用于开展中试规模的田间试验，目标是不育雄虫与可育雄虫的比例达到5比1。
