Innovation Research Institute of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, China.
Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine, Shenzhen, Guangdong, China.
Biomed Pharmacother. 2023 Jul;163:114755. doi: 10.1016/j.biopha.2023.114755. Epub 2023 Apr 25.
Vascular inflammation is a common pathological basis underlying many cardiovascular diseases. As such, the treatment of vascular inflammation has attracted increasing attention. The Piezo1 pathway has long been shown to play an important role in the development of vascular inflammation. Jatrorrhizine (Jat) is an effective component of Rhizoma Coptidis. It is commonly used in the treatment of inflammatory diseases and is a potential drug for the treatment of vascular inflammation. However, its mechanism of action on vascular inflammation remains unclear, as is the effect of Jat on Piezo1. Therefore, we conducted a series of studies on the effect of jatrorrhizine on vascular inflammation in vivo and in vitro. In this study, the effect of Jat treatment on HO-induced endothelial cell inflammation was investigated in vitro, and the potential mechanism of Jat was explored. In in vivo experiments, we investigated the effect of jatrorrhizine on vascular inflammation induced by carotid artery ligation and its effect on the Piezo1 signaling pathway. We found that Jat could reduce the severity of carotid intimal hyperplasia and local vascular inflammation in mice. In the HO-induced inflammation model, cell proliferation and migration were significantly inhibited, and the expression of pro-inflammatory factors was reduced. Importantly, the addition of Jat to endothelial Piezo1 knockout did not produce further significant inhibition. We believe that the role of Jat in the treatment of vascular inflammation may be related to Piezo1. And we believe that Jat has great potential in the treatment of vascular inflammation and cardiovascular diseases.
血管炎症是许多心血管疾病的共同病理基础。因此，血管炎症的治疗引起了越来越多的关注。Piezo1 通路长期以来一直被认为在血管炎症的发展中起着重要作用。小檗碱（Jat）是黄连根茎的有效成分之一。它常用于治疗炎症性疾病，是治疗血管炎症的潜在药物。然而，其对血管炎症的作用机制尚不清楚，Jat 对 Piezo1 的作用也是如此。因此，我们进行了一系列关于小檗碱在体内和体外对血管炎症影响的研究。在这项研究中，我们研究了 Jat 处理对 HO 诱导的内皮细胞炎症的影响，并探讨了 Jat 的潜在机制。在体内实验中，我们研究了小檗碱对颈动脉结扎诱导的血管炎症的影响及其对 Piezo1 信号通路的影响。我们发现小檗碱可以减轻小鼠颈动脉内膜增生和局部血管炎症的严重程度。在 HO 诱导的炎症模型中，细胞增殖和迁移明显受到抑制，促炎因子的表达减少。重要的是，将 Jat 添加到内皮细胞 Piezo1 敲除中并没有产生进一步的显著抑制作用。我们认为 Jat 在治疗血管炎症中的作用可能与 Piezo1 有关。我们相信 Jat 在治疗血管炎症和心血管疾病方面具有巨大的潜力。
