Southeast Dairy Foods Research Center, North Carolina State University, Raleigh 27695.
Northeast Dairy Foods Research Center, Department of Food Science, Cornell University, Ithaca, NY 14853.
J Dairy Sci. 2023 Jun;106(6):3884-3899. doi: 10.3168/jds.2022-22887. Epub 2023 Apr 25.
Our objective was to determine the effect of addition of dipotassium phosphate (DKP) at 3 different thermal treatments on color, viscosity, and sensory properties of 7.5% milk protein-based beverages during 15 d of storage at 4°C. Micellar casein concentrate (MCC) and milk protein concentrate (MPC) containing about 7.5% protein were produced from pasteurized skim milk using a 3×, 3-stage ceramic microfiltration process and a 3×, 3-stage polymeric ultrafiltration membrane process, respectively. The MCC and MPC were each split into 6 batches, based on thermal process and addition of DKP. The 6 batches were no postfiltration heat treatment with added DKP (0.15%), no postfiltration heat without added DKP (0%), postfiltration high-temperature, short time (HTST) with DKP, postfiltration HTST without DKP, postfiltration direct steam injection with DKP, and postfiltration direct steam injection without DKP. The 6 MCC milk-based beverages and the 6 MPC milk-based beverages were stored at 4°C. Viscosity, color, and sensory properties were determined over 15 d of refrigerated storage. MCC- and MPC-based beverages at 7.5% protein with and without 0.15% added dipotassium phosphate were successfully run through an HTST and direct steam injection thermal process. The 7.5% protein MCC-based beverage contained a higher calcium and phosphorus content (2,425 and 1,583 mg/L, respectively) than the 7.5% protein MPC-based beverages (2,141 and 1,338 mg/L, respectively). Pasteurization (HTST) had very little effect on beverage particle size distribution, whereas direct steam injection thermal processing produced protein aggregates with medians in the range of 10 and 175 μm for MPC beverages. A population of casein micelles at about 0.15 μm was found in both MCC- and MPC-based beverages. Larger particles in the 175-μm range were not detected in the MCC beverages. In general, the apparent viscosity (AV) of MCC beverages was higher than MPC beverages. Added DKP increased the AV of both MCC- and MPC-based beverages, while increasing heat treatment decreased AV. The AV of beverages with DKP increased during 15 d of 4°C of storage for both MCC and MPC, whereas there was very little change in AV during storage without DKP and a similar effect was observed for sensory viscosity scores. The L value of beverages was higher with higher heat treatment, but DKP addition decreased L value and sensory opacity greatly. Sulfur-eggy flavors were detected in MPC beverages, but not MCC-based beverages.
我们的目标是确定在 4°C 下储存 15 天时,添加磷酸氢二钾(DKP)在 3 种不同热处理中的效果对 7.5%牛奶蛋白基饮料的颜色、粘度和感官特性的影响。使用 3×3 阶段陶瓷微滤工艺和 3×3 阶段聚合超滤膜工艺,从巴氏杀菌脱脂乳中生产乳清浓缩蛋白(MCC)和乳蛋白浓缩物(MPC),蛋白质含量约为 7.5%。MCC 和 MPC 各分为 6 批,基于热过程和 DKP 的添加。6 批未进行过滤后热处理并添加 DKP(0.15%)、未进行过滤后热处理且未添加 DKP(0%)、过滤后高温短时间(HTST)加 DKP、过滤后 HTST 无 DKP、过滤后直接蒸汽喷射加 DKP 和过滤后直接蒸汽喷射无 DKP。6 种 MCC 牛奶基饮料和 6 种 MPC 牛奶基饮料在 4°C 下储存。在冷藏储存 15 天的过程中,测定了粘度、颜色和感官特性。含有和不含有 0.15%添加二磷酸氢钾的 7.5%蛋白质 MCC 和 MPC 基饮料成功通过 HTST 和直接蒸汽喷射热工艺运行。7.5%蛋白质 MCC 基饮料的钙和磷含量(分别为 2425 和 1583mg/L)高于 7.5%蛋白质 MPC 基饮料(分别为 2141 和 1338mg/L)。巴氏杀菌(HTST)对饮料粒径分布几乎没有影响,而直接蒸汽喷射热加工产生了 MPC 饮料中中值在 10 到 175μm 之间的蛋白质聚集体。在 MCC 和 MPC 基饮料中都发现了约 0.15μm 的酪蛋白胶束群体。在 MCC 饮料中未检测到 175μm 范围内的较大颗粒。一般来说,MCC 饮料的表观粘度(AV)高于 MPC 饮料。添加 DKP 增加了 MCC 和 MPC 基饮料的 AV,而增加热处理则降低了 AV。在 4°C 下储存 15 天期间,添加 DKP 的 MPC 和 MCC 饮料的 AV 均增加,而在不添加 DKP 的情况下 AV 变化很小,并且对感官粘度评分也有类似的影响。随着热处理温度的升高,饮料的 L 值升高,但 DKP 的添加大大降低了 L 值和感官不透明度。在 MPC 饮料中检测到硫-鸡蛋味,但在 MCC 基饮料中未检测到。