Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong University, Cooperative Innovation Center of Sustainable Pig Production, Wuhan 430070, China.
Int J Mol Sci. 2023 Apr 18;24(8):7442. doi: 10.3390/ijms24087442.
() is one of the most important zoonotic pathogens that threaten the lives of pigs and humans. Even worse, the increasingly severe antimicrobial resistance in is becoming a global issue. Therefore, there is an urgent need to discover novel antibacterial alternatives for the treatment of infection. In this study, we investigated theaflavin (TF1), a benzoaphenone compound extracted from black tea, as a potential phytochemical compound against . TF1 at MIC showed significant inhibitory effects on growth, hemolytic activity, and biofilm formation, and caused damage to cells in vitro. TF1 had no cytotoxicity and decreased adherent activity of to the epithelial cell Nptr. Furthermore, TF1 not only improved the survival rate of -infected mice but also reduced the bacterial load and the production of IL-6 and TNF-α. A hemolysis test revealed the direct interaction between TF1 and Sly, while molecular docking showed TF1 had a good binding activity with the Glu198, Lys190, Asp111, and Ser374 of Sly. Moreover, virulence-related genes were downregulated in the TF1-treated group. Collectively, our findings suggested that TF1 can be used as a potential inhibitor for treating infection in view of its antibacterial and antihemolytic activity.
（）是一种重要的人畜共患病病原体，威胁着猪和人类的生命。更糟糕的是，（）日益严重的抗药性问题正在成为一个全球性问题。因此，迫切需要发现治疗（）感染的新型抗菌替代品。在这项研究中，我们研究了茶黄素（TF1），一种从红茶中提取的苯并二氢吡喃酮化合物，作为一种潜在的植物化学化合物来对抗（）。MIC 下的 TF1 对（）的生长、溶血活性和生物膜形成表现出显著的抑制作用，并在体外对（）细胞造成损伤。TF1 没有细胞毒性，并降低了（）对上皮细胞 Nptr 的黏附活性。此外，TF1 不仅提高了感染（）的小鼠的存活率，还降低了细菌负荷和 IL-6 和 TNF-α 的产生。溶血试验表明 TF1 与 Sly 之间存在直接相互作用，而分子对接表明 TF1 与 Sly 的 Glu198、Lys190、Asp111 和 Ser374 具有良好的结合活性。此外，在 TF1 处理组中，毒力相关基因下调。总的来说，我们的研究结果表明，鉴于 TF1 的抗菌和抗溶血活性，它可以用作治疗（）感染的潜在抑制剂。
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