Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture and Rural Affairs), Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan, China.
Institute of Fruit and Tea, Hubei Academy of Agricultural Sciences, Wuhan, China.
Front Cell Infect Microbiol. 2022 Sep 20;12:973282. doi: 10.3389/fcimb.2022.973282. eCollection 2022.
() is a highly virulent zoonotic pathogen and causes severe economic losses to the swine industry worldwide. Public health security is also threatened by the rapidly growing antimicrobial resistance in . Therefore, there is an urgent need to develop new and safe antibacterial alternatives against . The green tea polyphenol epigallocatechin gallate (EGCG) with a number of potential health benefits is known for its antibacterial effect; however, the mechanism of its bactericidal action remains unclear. In the present, EGCG at minimal inhibitory concentration (MIC) showed significant inhibitory effects on growth, hemolytic activity, and biofilm formation, and caused damage to cells . EGCG also reduced pathogenicity in larvae . Metabolomics and proteomics analyses were performed to investigate the underlying mechanism of antibacterial activity of EGCG at MIC. Many differentially expressed proteins involved in DNA replication, synthesis of cell wall, and cell membrane, and virulence were down-regulated after the treatment of with EGCG. EGCG not only significantly reduced the hemolytic activity of but also down-regulated the expression of suilysin (Sly). The top three shared KEGG pathways between metabolomics and proteomics analysis were ABC transporters, glycolysis/gluconeogenesis, and aminoacyl-tRNA biosynthesis. Taken together, these data suggest that EGCG could be a potential phytochemical compound for treating infection.
（）是一种高毒力的人畜共患病病原体，在全球范围内给养猪业造成严重的经济损失。（）中迅速增长的抗菌药物耐药性也威胁着公共卫生安全。因此，迫切需要开发针对（）的新型安全抗菌替代品。具有多种潜在健康益处的绿茶多酚表没食子儿茶素没食子酸酯（EGCG）以其抗菌作用而闻名；然而，其杀菌作用的机制尚不清楚。在本研究中，最小抑菌浓度（MIC）的 EGCG 对（）的生长、溶血活性和生物膜形成表现出显著的抑制作用，并导致（）细胞损伤。EGCG 还降低了（）幼虫的致病性。进行代谢组学和蛋白质组学分析，以研究 MIC 下 EGCG 的抗菌活性的潜在机制。用 EGCG 处理后，许多参与 DNA 复制、细胞壁和细胞膜合成以及毒力的差异表达蛋白下调。EGCG 不仅显著降低了（）的溶血活性，还下调了溶血素（Sly）的表达。代谢组学和蛋白质组学分析之间的前三个共同 KEGG 途径是 ABC 转运蛋白、糖酵解/糖异生和氨酰-tRNA 生物合成。综上所述，这些数据表明，EGCG 可能是治疗（）感染的潜在植物化学化合物。
