终末期肾病患者透析开始前后B淋巴细胞亚群的前瞻性分析

Prospective Analysis of B Lymphocyte Subtypes, before and after Initiation of Dialysis, in Patients with End-Stage Renal Disease.

作者信息

Daikidou Dimitra-Vasilia, Lioulios Georgios, Sampani Erasmia, Xochelli Aliki, Nikolaidou Vasiliki, Moysidou Eleni, Christodoulou Michalis, Iosifidou Artemis, Iosifidou Myrto, Briza Dimitria Ioanna, Papagianni Aikaterini, Fylaktou Asimina, Stangou Maria

机构信息

School of Medicine, Aristotle University of Thessaloniki, 45636 Thesaloniki, Greece.

Department of Nephrology, Hippokration Hospital, 54642 Thessaloniki, Greece.

出版信息

Life (Basel). 2023 Mar 23;13(4):860. doi: 10.3390/life13040860.

DOI:10.3390/life13040860

PMID:37109388

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10146774/

Abstract

UNLABELLED

End-stage renal disease (ESRD) is followed by alterations in adaptive immunity. The aim of this study was to evaluate B lymphocyte subtypes in ESRD patients before and after hemodialysis (HD) or continuous ambulatory peritoneal dialysis (CAPD).

PATIENTS AND METHODS

CD5, CD27, BAFF, IgM and annexin were evaluated by flow cytometry on CD19+ cells in ESRD patients (n = 40), at time of initiating HD or CAPD (T0) and 6 months later (T6).

RESULTS

A significant reduction in ESRD-T0 compared to controls was noticed for CD19+, 70.8 (46.5) vs. 171 (249), < 0.0001, CD19+CD5-, 68.6 (43) vs. 168.9 (106), < 0.0001, CD19+CD27-, 31.2 (22.1) vs. 59.7 (88.4), < 0.0001, CD19+CD27+, 42.1 (63.6) vs. 84.3 (78.1), = 0.002, CD19+BAFF+, 59.7 (37.8) vs. 127.9 (123.7), < 0.0001 and CD19+IgM+ cells, 48.9 (42.8) vs. 112.5 (81.7) (K/μL), < 0.0001. The ratio of early/late apoptotic B lymphocytes was reduced (16.8 (10.9) vs. 110 (25.4), = 0.03). CD19+CD5+ cells were the only cell type with an increased proportion in ESRD-T0 patients (2.7 (3.7) vs. 0.6 (1.1), < 0.0001). After 6 months on CAPD or HD, CD19+CD27-(%) and early apoptotic lymphocytes were reduced further. The HD patients also showed a significant increase in late apoptotic lymphocytes, from 1.2 (5.7) to 4.2 (7.2) K/mL, = 0.02.

CONCLUSIONS

B cells and most of their subtypes were significantly reduced in ESRD-T0 patients compared to controls, the only exception being CD19+CD5+ cells. Apoptotic changes were prominent in ESRD-T0 patients and were exacerbated by HD.

摘要

未标记

终末期肾病（ESRD）会伴随适应性免疫的改变。本研究的目的是评估血液透析（HD）或持续性非卧床腹膜透析（CAPD）前后ESRD患者的B淋巴细胞亚群。

患者与方法

通过流式细胞术对40例ESRD患者（HD或CAPD起始时（T0）及6个月后（T6））的CD19⁺细胞进行CD5、CD27、BAFF、IgM和膜联蛋白评估。

结果

与对照组相比，ESRD - T0时CD19⁺细胞显著减少，分别为70.8（46.5）对171（249），<0.0001；CD19⁺CD5⁻细胞，68.6（43）对168.9（106），<0.0001；CD19⁺CD27⁻细胞，31.2（22.1）对59.7（88.4），<0.0001；CD19⁺CD27⁺细胞，42.1（63.6）对84.3（78.1），=0.002；CD19⁺BAFF⁺细胞，59.7（37.8）对127.9（123.7），<0.0001；CD19⁺IgM⁺细胞，48.9（42.8）对112.5（81.7）（K/μL），<0.0001。早期/晚期凋亡B淋巴细胞的比例降低（16.8（10.9）对110（25.4），=0.03）。CD19⁺CD5⁺细胞是ESRD - T0患者中比例增加的唯一细胞类型（2.7（