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银粒子包覆的壳聚糖/明胶复合膜与双层膜的抗菌、生物降解及生物相容性性能的比较研究

Comparative Study of the Antibacterial, Biodegradable, and Biocompatibility Properties of Composite and Bi-Layer Films of Chitosan/Gelatin Coated with Silver Particles.

作者信息

Valencia-Gómez Laura-Elizabeth, Reyes-Blas Hortensia, Hernández-Paz Juan-Francisco, Rodríguez-González Claudia-Alejandra, Olivas-Armendáriz Imelda

机构信息

Instituto de Ingeniería y Tecnología, Universidad Autónoma de Ciudad Juárez, Ave. Del Charro #610 Norte, Col. Partido Romero, Cd. Juárez 32320, Mexico.

出版信息

Materials (Basel). 2023 Apr 10;16(8):3000. doi: 10.3390/ma16083000.

Abstract

The dressings are materials that can improve the wound-healing process in patients with medical issues. Polymeric films are frequently used as dressings with multiple biological properties. Chitosan and gelatin are the most used polymers in tissue regeneration processes. There are usually several configurations of films for dressings, among which the composite (mixture of two or more materials) and layered ones stand out (layers). This study analyzed the antibacterial, degradable, and biocompatible properties of chitosan and gelatin films in 2 configurations, composite and bilayer, composite. In addition, a silver coating was added to enhance the antibacterial properties of both configurations. After the study, it was found that the bilayer films have a higher antibacterial activity than the composite films, having inhibition halos between 23% and 78% in Gram-negative bacteria. In addition, the bilayer films increased the fibroblast cell proliferation process, reaching up to 192% cell viability after 48 h of incubation. On the other hand, composite films have greater stability since they are thicker, with 276 µm, 243.8 µm, and 239 µm compared to 236 µm, 233 µm, and 219 µm thick for bilayer films; and a low degradation rate compared to bilayer films.

摘要

敷料是能够改善有医疗问题患者伤口愈合过程的材料。聚合物薄膜常被用作具有多种生物学特性的敷料。壳聚糖和明胶是组织再生过程中最常用的聚合物。用于敷料的薄膜通常有几种构型,其中复合(两种或更多材料的混合物)和分层的薄膜最为突出。本研究分析了壳聚糖和明胶薄膜在复合和双层这两种构型下的抗菌、可降解和生物相容性特性。此外,添加了银涂层以增强两种构型的抗菌性能。研究后发现,双层薄膜比复合薄膜具有更高的抗菌活性,对革兰氏阴性菌的抑菌圈在23%至78%之间。此外,双层薄膜促进了成纤维细胞增殖过程,孵育48小时后细胞活力高达192%。另一方面,复合薄膜更稳定,因为它们更厚,分别为276 µm、243.8 µm和239 µm,而双层薄膜的厚度为236 µm、233 µm和219 µm;并且与双层薄膜相比降解率较低。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef74/10144850/a7c749c245e4/materials-16-03000-g001.jpg

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