Duplessis Mélissa, Chorfi Younes, Girard Christiane L
Sherbrooke Research and Development Centre, Agriculture & Agri-Food Canada, Sherbrooke, QC J1M 0C8, Canada.
Département de biomédecine vétérinaire, Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, QC J2S 2M2, Canada.
Metabolites. 2023 Apr 11;13(4):547. doi: 10.3390/metabo13040547.
It is well established that the plasma metabolite profile changes during metabolic dysfunction, such as elevated non-esterified fatty acid (NEFA) release when body reserve mobilization is excessive in early lactation cows. Relationships between changes in plasma concentrations of metabolites caused by a metabolic impairment and the status of vitamins, such as folates and vitamin B, have barely been studied in cattle. This study was undertaken to assess relationships between peripartum plasma concentrations of folates, vitamin B, NEFA, and beta-hydroxybutyrate (BHB). Longitudinal data of 48 multiparous Holstein cows from 5 studies were taken from days -14 to 21 relative to calving. Blood samples were taken weekly before calving and either twice or thrice per week postpartum, and plasma was analyzed for folate, vitamin B, NEFA, and BHB concentrations. Postpartum plasma NEFA and BHB concentrations were negatively related to plasma folate concentration at days -14 and -7 relative to parturition, whereas the opposite relationship was noted for the plasma vitamin B:folate ratio. The plasma folate and NEFA areas under the curve from the whole studied period were negatively associated, and the opposite was observed with the association between the plasma vitamin B:folate ratio and NEFA as well as the BHB areas under the curve. The results suggest that there is an increased use of folate for metabolic functions during elevated concentrations of plasma NEFA and BHB. Future research should focus on finding an optimal plasma vitamin B:folate ratio to favor cow health during the challenging period of parturition.
众所周知，在代谢功能障碍期间血浆代谢物谱会发生变化，例如在泌乳早期奶牛体内储备动员过度时非酯化脂肪酸（NEFA）释放增加。由代谢损伤引起的血浆代谢物浓度变化与维生素（如叶酸和维生素B）状态之间的关系在牛身上几乎未被研究过。本研究旨在评估围产期血浆叶酸、维生素B、NEFA和β-羟基丁酸（BHB）浓度之间的关系。从5项研究中选取了48头经产荷斯坦奶牛在产犊前14天至产后21天的纵向数据。在产犊前每周采集血样，产后每周采集两次或三次，分析血浆中叶酸、维生素B、NEFA和BHB的浓度。产后血浆NEFA和BHB浓度与分娩前14天和7天的血浆叶酸浓度呈负相关，而血浆维生素B:叶酸比值则呈现相反的关系。整个研究期间血浆叶酸和NEFA曲线下面积呈负相关，血浆维生素B:叶酸比值与NEFA以及BHB曲线下面积之间的关系则相反。结果表明，在血浆NEFA和BHB浓度升高期间，叶酸用于代谢功能的量增加。未来的研究应集中在寻找最佳的血浆维生素B:叶酸比值，以在产犊这一具有挑战性的时期促进奶牛健康。
